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Hochhalter et al. Association between HCMV and GBM

Authors Number of samples analyzed Detection method Analyte
[53]
Cosset et al., 2014 0/20 GBMs Semi-qPCR for CMV RNA, DNA
Nested PCR for gB
0/5 GBMs RNA-seq RNA
[34]
Yamashita et al., 2014 0/59 GBMs (40 fresh-frozen and 19 PCR for gB and IE1 DNA
FFPE)
0 (confirmed)/5 GBMs (false- WB for IE1/2 and pp28 Protein
positive staining on WB confirmed
by LC/MS/MS analysis)
10/10 GBMs IHC for pp28 Protein
7/10 GBMs IHC for IE1/2
5/10 GBMs IHC for pp65
0/10 GBMs IHC for UL44
0/10 GBMs FISH HCMV BAC DNA DNA
[54]
Solomon et al., 2014 0/68 GBM TMA IHC for HCMV cocktail Protein
[10]
Baumgarten et al., 2014 0/91 GBMs FFPE IHC for p52, pp65, IEA Protein
0/10 GBMs PCR for HCMV loci DNA
Libard et al., [55] 2014 363/417 extra- and intra-axial brain IHC for pp65 Protein
tumors (61/68 GBMs) TMA
[56]
Ahani et al., 2014 0/8 non-glioma tumor tissue PCR (HCMV detection kit) DNA
0/2 PA
1/3 AA
4/7 OA
12/16 GBMs
Tang et al., [57] 2015 0/34 GBM WGS DNA
[58]
Wakefiled et al., 2015 14/24 peds GBMs IHC for IE1-72 DNA, Protein
12/24 peds GBMs IHC for pp65
13/16 peds GBMs ISH for HCMV DNA probe cocktail
[59]
Bianchi et al., 2015 30/43 GBMs fresh frozen IF for IE1 and LA Protein
8/14 ODG
17/20 meningiomas
2/6 IE1 IF-positive GBMs IHC for IE1 Protein
17/34 GBMs Nested PCR for gB DNA
5/14 ODG
6/13 meningiomas
[60]
Shamran et al., 2015 33/36 GBM FFPE IHC for IE1-72 DNA, Protein
28/36 GBM FFPE IHC for pp65
26/36 GBM FFPE IHC for LA
10/10 selected GBM samples Nested PCR for IE1
0/30 meningioma FFPE IHC for IE1-72, pp65, and late antigen
Holdhoff et al., [35] 2016 0/25 GBMs fresh-frozen qPCR for US17 DNA, RNA,
0/70 HGG TMA IHC for pp65 Protein
0/20 GBMs FFPE IHC and CISH for IE1/2 and pp65
3/18 HCMV DNA plasma samples
8/15 serum HCMV IgG
0/18 GBMs FFPE IHC, CISH, PCR
[36]
Strong et al., 2016 0/157 GBM RNA-seq datasets RNA
0/13 recurrent GBM
0/514 LGGs
0/17 recurrent LGGs
0/92 MRI-guided GBM biopsies
0/9 glioma stem-like cell cultures
0/51 GBM WGS datasets DNA
0/10 recurrent GBM
0/64 meningioma
[61]
Lin et al., 2016 0/19 GBM FFPE, 0/20 GBM OCT, ddPCR for HCMV UL55 DNA
0/6 GBM fresh frozen
4/19 GBM FFPE, 0/20 GBM OCT, ddPCR for EBV LMP1
0/6 GBM fresh frozen
3/19 GBM FFPE, 3/20 GBM OCT,
0/6 GBM fresh frozen ddPCR for HHV-6 U57
[62]
Taha et al., 2016 0/32 GBMs IHC for HCMV and PCR for UL34, UL80.5 DNA, Protein
[63]
Stangherlin et al., 2016 38/52 GBMs PCR for UL83 DNA, Protein
30/52 GBMs IHC for HCMV nuclear protein
19/52 GBMs ISH for early 2.7 RNA
[25]
Xing et al., 2016 52/79 glioma IHC for pp65 DNA, Protein
43/79 glioma ISH for pp65 DNA
FFPE: formalin-fixed paraffin-embedded; IHC: immunohistochemistry; ISH: in situ hybridization; HCMV: human cytomegalovirus; PCR:
polymerase chain reaction; GBM: glioblastoma multiforme; EM: electron microscopy; ODG: oligodendroglioma; CNS: central nervous
system; TMA: tissue microarray; ACN: acoustic neuromas; AA: anaplastic astrocytoma; LGG: low-grade glioma; WB: western blot; LA:
HCMV late antigen; FISH: fluorescence in situ hybridization; IF: immunofluorescence; PA: pilocystic astrocytoma; OA: oligoastrocytoma;
WGS: whole genome sequencing; CISH: chromogenic in situ hybridization; ddPCR: digital droplet PCR; OCT: optimal cutting temperature;
HGG: high-grade glioma; IEA: immediate-early antigen
100 Neuroimmunology and Neuroinflammation ¦ Volume 4 ¦ June 16, 2017