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Hochhalter et al. Association between HCMV and GBM

Table 1: Studies evaluating presence of HCMV in gliomas
Authors Number of samples analyzed Detection method Analyte
[3]
Cobbs et al., 2002 27/27 gliomas FFPE IHC for IE1-72, pp65, p52 Protein
10/10 gliomas ISH biotinylated oligonucleotide probe RNA, DNA
specific for HCMV early gene mRNA
ISH digoxigenin-labeled HCMV total
genome DNA probe
7/9 gliomas Nested PCR for gB (UL55) DNA
2/2 GBM EM-IHC anti-pp65 mAb and gold particles Viral particles
Lau et al., [37] 2005 0/17 gliomas FFPE IHC for HCMV cocktail, pp65 Protein
0/2 ODG FFPE
0/3 ependymomas FFPE
0/17 gliomas FFPE ISH digoxigenin-labeled probe specific for RNA, DNA
0/2 ODG FFPE HCMV early gene mRNA
0/3 ependymomas FFPE ISH digoxigenin-labeled probe specific for
HCMV DNA (pp65 and pp150)
0/17 gliomas FFPE PCR for gB (UL55) DNA
0/2 ODG FFPE
0/3 ependymomas FFPE
Sabatier et al., 2005 9/116 CNS tumors (15 IHC for IE1, p52 DNA, Protein
[38]
ependymomas, 81 GBM, 20 ODG) ISH biotinylated HCMV DNA probe
TMA
1/25 gliomas fresh frozen
[39]
Poltermann et al., 2006 0/73 CNS tumors (38 gliomas, 29 IHC for IE, EA, pp65 Protein
meningiomas, 6 ACNs) FFPE
0/77 (40 gliomas, 31 meningiomas, Nested PCR for IE1 and gB (UL55) DNA
6 ACNs)
[40]
Mitchell et al., 2008 42/45 GBMs FFPE IHC for IE1-72 Protein
30/33 GBMs FFPE IHC for pp65
16/16 GBMs FFPE ISH FITC-conjugated 40-mer probes for DNA
HCVM IE1
ISH biotinylated HCMV total genome DNA
probe
16/17 GBM primary cultures IHC for IE1, pp65 gB, and pp28
21/34 GBMs fresh frozen PCR for gB (UL55) DNA
13/17 GBM primary cultures
Scheurer et al., 2008 21/21 GBM FFPE IHC for IE1-72 and ISH fluorescein-labeled Protein, DNA
[41]
9/12 AA FFPE oligonucleotide total HCMV genome DNA
14/17 LGG FFPE probe mixture
Slinger et al., 2010 20/21 gliomas FFPE IHC for IEA, US28 Protein
[29]
[42]
Lucas et al., 2011 25/49 GBMs FFPE IHC for pp65 Protein
8/49 GBMs FFPE IHC for IE1
[43]
Ranganathan et al., 2012 75/75 GBM FFPE PCR using 12 HCMV primer pairs DNA
12/12 GBM fresh frozen PCR using 19 HCMV primer pairs
[44]
Rahbar et al., 2012 79/80 GBMs FFPE IHC for IEA Protein
76/80 GBMs FFPE IHC for LA
6/6 selected GBM FFPE ISH HCMV-DNA total genome fluorescein DNA
labeled probes
[45]
Bhattacharjee et al., 2012 16/17 gliomas fresh frozen Nested PCR for IE DNA
9/12 gliomas fresh frozen WB for pp65, IE1-72, gB Protein
[46]
Fonseca et al., 2012 27/75 gliomas fresh frozen PCR for pp65 DNA
[47]
Khoury et al., 2013 0/168 GBMs RNA-seq RNA
0/47 LGGs
[48]
Tang et al., 2013 0/167 GBMs RNA-seq RNA
[49]
Rahbar et al., 2013 74/75 GBMs FFPE IHC for IEA Protein
70/75 GBMs FFPE IHC for LA
19/19 selected GBM FFPE ISH CMV DNA probe DNA
5/5 GBM primary cultures PCR for IE DNA
[50]
Ding et al., 2014 51/67 gliomas FFPE IHC for IE1-72 Protein
44/67 gliomas FFPE IHC for pp65
35/67 gliomas Nested PCR for gB (UL55) DNA
[51]
Dos Santos et al., 2014 21/22 GBMs fresh frozen PCR for pp65 DNA
20/22 GBMs fresh frozen hemi-nested PCR for gB (UL55)
Cimino et al., 2014 0/21 gliomas Unmapped reads from targeted cancer DNA
[52]
gene panel
Continued...
Neuroimmunology and Neuroinflammation ¦ Volume 4 ¦ June 16, 2017 99