Page 98 - Read Online

Amatori et al. J Cancer Metastasis Treat 2017;3:90-9 Journal of
DOI: 10.20517/2394-4722.2017.22
Cancer Metastasis and Treatment

www.jcmtjournal.com
Original Article Open Access

Real-time quantitative PCR array to study

drug-induced changes of gene expression

in tumor cell lines

Stefano Amatori , Giuseppe Persico , Mirco Fanelli 1
1,2
1
1 Molecular Pathology Laboratory “PaoLa”, Department of Biomolecular Sciences, University of Urbino “Carlo Bo”, 61032 Fano (PU), Italy.
2 Department of Experimental Oncology, European Institute of Oncology, 20139 Milan, Italy.

Correspondence to: Dr. Mirco Fanelli, Molecular Pathology Laboratory “PaoLa”, Department of Biomolecular Sciences, University of Urbino
“Carlo Bo”, 61032 Fano (PU), Italy. E-mail: mirco.fanelli@uniurb.it
How to cite this article: Amatori S, Persico G, Fanelli M. Real-time quantitative PCR array to study drug-induced changes of gene expression in
tumor cell lines. J Cancer Metastasis Treat 2017;3:90-9.
ABSTRACT
Article history: Aim: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is still the
Received: 10-04-2017 “gold standard” for quantitative analysis of mRNA and the study of differentially expressed
Accepted: 26-04-2017 genes. Methods: The authors describe a RT-qPCR array that exploits SYBR Green dye-
Published: 24-05-2017 based detection to perform reliable gene expression analysis on 41 genes involved in several
pathways linked to DNA damage response, cell cycle progression, cellular senescence,
Key words: and programmed cell death. To validate the RT-qPCR array, the authors investigated
Reverse transcription-quantitative changes of the gene expression profile of HeLa cells treated with two well-characterized
polymerase chain reaction, antiproliferative molecules such as cisplatin (CDDP) and sodium butyrate (NaBu). Results:
gene expression, The results showed a gene expression profile compatible with both biological and gene
cancer treatment
expression data already reported in literature. Conclusion: Importantly, the assay allowed
the monitoring of additional and not reported gene regulations, indicating that this custom-
made RT-qPCR array is a cheap, robust, and rapid tool for the study of drug-induced effects
in human biological models.

INTRODUCTION treatment efficacy. [1]

The study of gene expression profile of cancer cells Reverse transcription-quantitative polymerase chain
has become an essential tool to understand the reaction (RT-qPCR)-based methods has emerged
biological alterations involved in disease development, as the “gold standard” method for a rapid and robust
to individuate new potential markers, to predict clinical analysis of gene expression. Currently, many PCR
[2]
outcome, to create personalized pharmacological arrays are commercially available for the study of
therapies for patients, and to investigate the molecular gene expression modifications involved in hundreds
effects of drug exposure with the aim of improving of molecular pathways. However, based on our

Quick Response Code:
This is an open access article distributed under the terms of the Creative Commons Attribution-
NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work
non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

For reprints contact: service@oaepublish.com

90 © 2017 OAE Publishing Inc. www.oaepublish.com