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Topic: Natural Products and Hepatocellular Carcinoma

Dietary incorporation of jojoba extract eliminates oxidative

damage in livers of rats fed fumonisin-contaminated diet

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Mosaad A. Abdel-Wahhab , Olivier Joubert , Aziza A. El-Nekeety , Hafiza A. Sharaf ,
Ferial M. Abu-Salem , Bertrand H. Rihn 2
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1 Food Toxicology and Contaminants Department, National Research Center, Dokki, Cairo 12311, Egypt.
2 Faculty of Pharmacy, EA 3452 CITHEFOR, Lorraine University, 54001 Nancy Cedex, France.
3 Department of Pathology, National Research Center, Dokki, Cairo 12311, Egypt.
4 Department of Food Science and Technology, National Research Center, Dokki, Cairo 12311, Egypt.

ABSTRACT
Aim: This study aimed to determine the composition of ethanol extract of jojoba seeds, and to evaluate its hepatoprotective
effects in rats fed fumonisin B (FB )-contaminated diet. Methods: Jojoba seeds were extracted in 95% ethanol, and the
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chemical composition was determined. Male rats were divided into six groups and treated for 8 weeks as follows: (1)
Untreated control; (2) FB -contaminated diet (80 mg/kg diet); (3) low dose (0.5 mg/kg b.w.) jojoba extract; (4) high dose (1.0
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mg/kg b.w.) jojoba extract; (5) low dose jojoba extract plus FB ; and (6) high dose jojoba extract plus FB . Blood and liver
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samples were collected for different biochemical analyses and histological examinations. Results: The results indicated that
the ethanolic extract of jojoba is rich in protein, phenolic compounds, phytic acid, and considerable amounts of simmondsin.
Animals fed FB -contaminated diet showed severe biochemical and histological changes typical to those reported in literature.
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Treatment with jojoba seed extract alone at the two tested doses did not induce significant alterations in all parameters tested.
Combined treatment of jojoba seed extract with FB eliminated hepatotoxicity induced by FB , especially at low dose of jojoba
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seed extract. Conclusion: The authors concluded that jojoba seed extract can be incorporated in FB -contaminated feed to
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eliminate FB -induced hepatotoxicity.
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Key words: Fumonisin B ; health hazards; jojoba seed; liver; mycotoxins; oxidative stress
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Address for correspondence:
Prof. Mosaad A. Abdel-Wahhab, Food Toxicology and Contaminants Department, National Research Center, Dokki, Cairo 12311, Egypt.
E-mail: mosaad_abdelwahhab@yahoo.com
Received: 19-07-2015, Accepted: 07-10-2015

INTRODUCTION Fumonisin B (FB ) is the most common toxin, which
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has been classified by the International Agency for
Fumonisins B (FBs) are mycotoxins produced by the Research on Cancer as a Group 2B carcinogen (possibly
[6]
fungal species Fusarium, including Fusarium verticillioides carcinogenic in humans). Long-term studies indicated
[7-9]
and Fusarium proliferatum. This mycotoxin is mainly that FB was hepatocarcinogenic in rats while another
[1,2]
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produced on corn and possibly sorghum, which study reported on the nephrocarcinogenicity and
[10,11]
remain the primary sources of human exposure. At cancer promoting activity in rats. Epidemiological
[3,4]
least 28 FBs have been isolated and characterized. This is an open access article distributed under the terms of the Creative
[5]
Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows
Access this article online others to remix, tweak, and build upon the work non-commercially, as long as the
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http://www.hrjournal.net/
How to cite this article: Abdel-Wahhab MA, Joubert O, El-Nekeety AA,
DOI: Sharaf HA, Abu-Salem FM, Rihn BH. Dietary incorporation of jojoba
10.4103/2394-5079.168078 extract eliminates oxidative damage in livers of rats fed fumonisin-
contaminated diet. Hepatoma Res 2016;2:78-86.

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