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Topic: Natural Products and Hepatocellular Carcinoma
Synergistic curative effect of chicory extract and cisplatin
Synergistic curative effect of chicory extract and cisplatin
against thioacetamide-induced hepatocellular carcinoma
against thioacetamide-induced hepatocellular carcinoma
Hanaa A. Hassan , Hanaa M . Serag , Nabil M. Abdel-Hamid , Mahmoud M. Amr 1
1
1
2
1 Department of Zoology, Division of Physiology, Faculty of Science, Mansoura University, Mansoura 35516, Egypt
2 Biochemistry Department, Pharmacy College, Kafr El-Sheikh University, Kafr El-Sheikh 33516, Egypt
ABSTRACT
Aim: Hepatocellular carcinoma (HCC) is the dominant form of primary liver cancer and is histologically and etiologically
distinct from other forms of primary liver cancer. The objective of this study was to elucidate the synergistic effect and the role
of chicory extract [inulin (IN)] as a chemo-sensitizer for cisplatin (CIS) treatment of HCC. Methods: Five groups of rats were
treated for 4 months. These groups consisted of the control group, a group receiving thioacetamide (TAA) (200 mg/kg b.w)
in drinking water, a group injected intraperitoneally with a single dose of CIS (7.5 mg/kg b.w) in addition to TAA for 4 months,
a group receiving oral doses of IN (10 mg/kg b.w) in addition to TAA for 4 months, and a group injected intraperitoneally
with a single dose of CIS (7.5 mg/kg b.w) and IN (10 mg/kg b.w) plus TAA for 4 months. Results: The current data exhibited
increment of serum and liver enzyme ( alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, creatine
kinase, and lactate dehydrogenase) activity, serum lipid profi le levels (total lipids, total cholesterol, triglycerides, low-density
lipoprotein, and very low-density lipoprotein), and a signifi cant increase in α-fetoprotein and bilirubin, accompanied with
reduced total serum protein and albumin levels in a HCC rat model. Histopathologically, numerous alterations were detected
in hepatic tissues of HCC rats, such as lymphocytic cell infi ltration, damage of hepatocytes, dilated congested central vein
with degenerated endothelial cells, and congested blood sinusoids in addition to Masson’s trichrome staining blue collagen
fi bers in hepatocytes and central vein indicating hepatic fi brosis. Treatment of HCC rats with CIS or IN improved such
deleterious effects, where IN is more effective than CIS, and the best effect can be observed in rats that received both CIS
and IN. Conclusion: It could be concluded that IN in chicory extract acts as a chemo-sensitizer to CIS for treatment in an
HCC rat model.
Key words: Chicory; cisplatin; hepatocellular carcinoma; inulin
Address for correspondence:
Dr. Hanaa A. Hassan, Department of Zoology, Division of Physiology, Faculty of Science, Mansoura University, Mansoura 35516, Egypt.
E-mail: drhanaahassan@yahoo.com
Received: 03-07-2015, Accepted: 23-09-2015
INTRODUCTION carcinoma (HCC), a major malignancy worldwide and is
increasingly associated with cancer-related death. HCC
[2]
Cancer is one of the most anxiety-inducing diseases and represents the most common primary malignancy of the
it is presenting with further prolongation and increasing liver. According to epidemiological surveys, the prevalence
occurrence. The most common and recurrent primary
[1]
malignancy of the liver is recognized as hepatocellular This is an open access article distributed under the terms of the Creative
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How to cite this article: Hassan HA, Serag HM, Abdel-Hamid NM,
DOI: Amr MM. Synergistic curative effect of chicory extract and cisplatin
10.4103/2394-5079.167376 against thioacetamide-induced hepatocellular carcinoma. Hepatoma Res
2015;1:147-54.
© 2015 Hepatoma Research | Published by Wolters Kluwer - Medknow 147