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Topic: Natural Products and Hepatocellular Carcinoma




          Synergistic curative effect of chicory extract and cisplatin
          Synergistic curative effect of chicory extract and cisplatin
          against thioacetamide-induced hepatocellular carcinoma
          against thioacetamide-induced hepatocellular carcinoma


          Hanaa A. Hassan , Hanaa M . Serag , Nabil M. Abdel-Hamid , Mahmoud M. Amr              1
                                                 1
                             1
                                                                           2
          1 Department of Zoology, Division of Physiology, Faculty of Science, Mansoura University, Mansoura 35516, Egypt
             2 Biochemistry Department, Pharmacy College, Kafr El-Sheikh University, Kafr El-Sheikh 33516, Egypt


               ABSTRACT
               Aim: Hepatocellular carcinoma (HCC) is the dominant form of primary liver cancer and is histologically and etiologically
               distinct from other forms of primary liver cancer. The objective of this study was to elucidate the synergistic effect and the role
               of chicory extract [inulin (IN)] as a chemo-sensitizer for cisplatin (CIS) treatment of HCC. Methods: Five groups of rats were
               treated for 4 months. These groups consisted of the control group, a group receiving thioacetamide (TAA) (200 mg/kg b.w)
               in drinking water, a group injected intraperitoneally with a single dose of CIS (7.5 mg/kg b.w) in addition to TAA for 4 months,
               a group receiving oral doses of IN (10 mg/kg b.w) in addition to TAA for 4 months, and a group injected intraperitoneally
               with a single dose of CIS (7.5 mg/kg b.w) and IN (10 mg/kg b.w) plus TAA for 4 months. Results: The current data exhibited
               increment of serum and liver enzyme (    alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, creatine
               kinase, and lactate dehydrogenase) activity, serum lipid profi le levels (total lipids, total cholesterol, triglycerides,   low-density
               lipoprotein, and very low-density lipoprotein), and a signifi cant increase in α-fetoprotein and bilirubin, accompanied with
               reduced total serum protein and albumin levels in a HCC rat model. Histopathologically, numerous alterations were detected
               in hepatic tissues of HCC rats, such as lymphocytic cell infi ltration, damage of hepatocytes, dilated congested central vein
               with degenerated endothelial cells, and congested blood sinusoids in addition to Masson’s trichrome staining blue collagen
               fi bers in hepatocytes and central vein indicating hepatic fi brosis. Treatment of HCC rats with CIS or IN improved such
               deleterious effects, where IN is more effective than CIS, and the best effect can be observed in rats that received both CIS
               and IN. Conclusion: It could be concluded that IN in chicory extract acts as a chemo-sensitizer to CIS for treatment in an
               HCC rat model.

               Key words: Chicory; cisplatin; hepatocellular carcinoma; inulin


          Address for correspondence:
          Dr. Hanaa A. Hassan, Department of Zoology, Division of Physiology, Faculty of Science, Mansoura University, Mansoura 35516, Egypt.
          E-mail: drhanaahassan@yahoo.com
          Received: 03-07-2015, Accepted: 23-09-2015



          INTRODUCTION                                        carcinoma (HCC), a major malignancy worldwide and is
                                                              increasingly associated with cancer-related death.  HCC
                                                                                                         [2]
          Cancer is one of the most anxiety-inducing diseases and   represents the most common primary malignancy of the
          it is presenting with further prolongation and increasing   liver. According to epidemiological surveys, the prevalence
          occurrence.  The most common and recurrent primary
                    [1]
          malignancy of the liver is recognized as hepatocellular   This is an open access article distributed under the terms of the Creative
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                                                                How to cite this article:  Hassan HA, Serag HM, Abdel-Hamid NM,
           DOI:                                                 Amr MM. Synergistic curative effect of chicory extract and cisplatin
           10.4103/2394-5079.167376                             against thioacetamide-induced hepatocellular carcinoma. Hepatoma Res
                                                                2015;1:147-54.


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