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Liu et al. Plast Aesthet Res 2016;3:322-7 Plastic and
DOI: 10.20517/2347-9264.2016.74
Aesthetic Research

www.parjournal.net
Original Article Open Access

Proliferation and adipogenic differentiation

of human adipose-derived stem cells

isolated from middle-aged patients with

prominent orbital fat in the lower eyelids

Guang-Peng Liu, Cai-He Liao, Yi-Pin Xu
Department of Plastic and Reconstructive Surgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.

Correspondence to: Dr. Guang-Peng Liu, Department of Plastic and Reconstructive Surgery, Shanghai Tenth People's Hospital, Tongji University
School of Medicine, Shanghai 200072, China. E-mail: guangpengliu@163.com

How to cite this article: Liu GP, Liao CH, Xu YP. Proliferation and adipogenic differentiation of human adipose-derived stem cells isolated from
middle-aged patients with prominent orbital fat in the lower eyelids. Plast Aesthet Res 2016;3:322-7.
ABSTRACT

Article history: Aim: To evaluate the age-related effects on the adipogenic differentiation and proliferation
Received: 07-09-2016 potentials of human orbital adipose-derived stem cells (OASCs). Methods: Orbital adipose
Accepted: 18-10-2016 samples were harvested from the central fat compartment in the lower eyelids of 10 young
Published: 25-10-2016 and middle-aged patients during routine blepharoplasty surgery. After assessment of the
morphological changes of adipocytes with aging, OASCs were isolated from the fat samples
Key words: and expanded in vitro. Differences in the stem cell colony number (fibroblast colony-forming
Orbital fat, unit), growth rate and phenotype characterization (flow cytometry analysis) were evaluated.
adipose-derived stem cells, The ability of OASCs to differentiate into adipocytes was determined by oil red O staining
progenitor cell number, and the mRNA expression level of peroxisome proliferator-activated receptor γ. Results: Fat
proliferation rate, cell size showed a decreasing trend with advancing age. Although no difference was found
adipogenic differentiation in the expression of cell surface markers, the colony number and proliferative rate of OASCs
from middle-aged donors were significantly lower than those from the young donors. The
adipogenic differentiation capacity of middle-aged OASCs was also reduced. These differences
were statistically significant (P < 0.001). Conclusion: The data showed that the progenitor
cell number, proliferation capacity and adipogenic potential of OASCs decreased with aging,
suggesting that using OASCs from elderly patients for therapeutic purposes might be restricted.

INTRODUCTION vitro, possess multi-lineage differentiation potential
[1]
and remain stable over long-term culture. Compared
Adipose-derived stem cells (ASCs) are a population to the adult stem cells derived from other tissue
of postnatal progenitor cells which exist abundantly sources, such as bone marrow, muscle and synovium,
in adipose tissue. These cells can expand rapidly in advantages of utilizing ASCs include the large

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