Page 328 - Read Online

Liu et al. Adipogenesis of obital fat stem cells

freshly isolated from OF were comparable between REFERENCES
two groups, the progenitor cell numbers declined in the
older group. Because some cell lines ceased to grow 1. De Francesco F, Ricci G, D’Andrea F, Nicoletti GF, Ferraro GA.
at passage 6, the cell growth kinetics were compared Human adipose stem cells: from bench to bedside. Tissue Eng Part B
from passages 1 to 5. It was found that the proliferation 2. Rev 2015;21:572-84.
Oedayrajsingh-Varma MJ, van Ham SM, Knippenberg M, Helder
rate of young OASCs was significantly greater than MN, Klein-Nulend J, Schouten TE, Ritt MJ, van Milligen FJ.
that of the middle-aged cells. Young OASCs could Adipose tissue-derived mesenchymal stem cell yield and growth
generally achieve more than 12 population doublings characteristics are affected by the tissue-harvesting procedure.
while 10 CPDs were the maximum that cells from Cytotherapy 2006;8:166-77.
middle-aged donors could achieve. Moreover, an age- 3. Schipper BM, Marra KG, Zhang W, Donnenberg AD, Rubin JP.
related decrease in the adipogenic-differentiation Regional anatomic and age effects on cell function of human
potential of OASCs was observed. Not only the 4. adipose-derived stem cells. Ann Plast Surg 2008;60:538-44.
Korn BS, Kikkawa DO, Hicok KC. Identification and characterization
percentage of oil red O staining-positive cells but also of adult stem cells from human orbital adipose tissue. Ophthal Plast
the mRNA expression of PPARγ, a key transcription Reconstr Surg 2009;25:27-32.
factor regulating adipogenic differentiation and 5. Chen SY, Mahabole M, Horesh E, Wester S, Goldberg JL, Tseng
maintaining fat cell phenotype, declined with age. SC. Isolation and characterization of mesenchymal progenitor
cells from human orbital adipose tissue. Invest Ophthalmol Vis Sci
In general, our data showed that aging had a great 2014;55:4842-52.
influence on the proliferation and differentiation 6. Ho JH, Ma WH, Tseng TC, Chen YF, Chen MH, Lee OK. Isolation
and characterization of multi-potent stem cells from human orbital
potentials of OASCs. The OASCs’ number, adipogenic fat tissues. Tissue Eng Part A 2011;17:255-66.
differentiation and proliferative potentials began 7. Lin KJ, Loi MX, Lien GS, Cheng CF, Pao HY, Chang YC, Ji AT, Ho
to decrease at middle or early old age, which may JH. Topical administration of orbital fat-derived stem cells promotes
render their use in autologous cell therapy unsuitable corneal tissue regeneration. Stem Cell Res Ther 2013;4:72.
in elderly patients. Whether other linage differentiation 8. Bujalska IJ, Durrani OM, Abbott J, Onyimba CU, Khosla P, Moosavi
capabilities of OASCs are correlated with age AH, Reuser TT, Stewart PM, Tomlinson JW, Walker EA, Rauz S.
remains to be investigated in further studies. On the Characterisation of 11 β-hydroxysteroid dehydrogenase 1 in human
orbital adipose tissue: a comparison with subcutaneous and omental
other side, our previous studies proved that ASCs fat. J Endocrinology 2007;192:279-88.
isolated from SF depots possess low immunogenicity 9. Lin TM, Tsai JL, Lin SD, Lai CS, Chang CC. Accelerated
and can maintain their biological functions after growth and prolonged lifespan of adipose tissue-derived human
cryopreservation. [15,16] If these properties prove true mesenchymal stem cells in a medium using reduced calcium and
in OASCs, allogenic or cryopresereved autologous antioxidants. Stem Cells Dev 2005;14:92-102.
OASCs may serve as alternatives for future clinical 10. Mendes SC, Tibbe JM, Veenhof M, Bakker K, Both S, Platenburg
applications in elderly patients. PP, Oner FC, de Bruijn JD, van Blitterswijk CA. Bone tissue-
engineered implants using human bone marrow stromal cells: effect
of culture conditions and donor age. Tissue Eng 2002;8:911-20.
Financial support and sponsorship 11. Chen HT, Lee MJ, Chen CH, Chuang SC, Chang LF, Ho ML,
This work was financially supported by the grants Hung SH, Fu YC, Wang YH, Wang HI, Wang GJ, Kang L, Chang
of National Natural Science Foundation of China JK. Proliferation and differentiation potential of human adipose-
(No. 31271027 and No. 81171475). derived mesenchymal stem cells isolated from elderly patients with
osteoporotic fractures. J Cell Mol Med 2012;16:582-93.
12. Zhu M, Kohan E, Bradley J, Hedrick M, Benhaim P, Zuk P. The effect
Conflicts of interest of age on osteogenic, adipogenic and proliferative potential of female
There are no conflicts of interest. adipose-derived stem cells. J Tissue Eng Regen Med 2009;3:290-301.
13. Billon N, Monteiro MC, Dani C. Developmental origin of adipocytes:
new insights into a pending question. Biol Cell 2008;100:563-75.
Patient consent 14. Kirkland JL, Dobson DE. Preadipocyte function and aging: links
All patients had previously given informed consent. between age-related changes in cell dynamics and altered fat tissue
function. J Am Geriatr Soc 1997;45:959-67.
Ethics approval 15. Liu G, Zhou H, Li Y, Li G, Cui L, Liu W, Cao Y. Evaluation of
The protocol for this study was approved by the the viability and osteogenic differentiation of cryopreserved human
adipose-derived stem cells. Cryobiology 2008;57:18-24.
Research Ethics Committee of Tongji University 16. Liu G, Zhang Y, Liu B, Sun J, Li W, Cui L. Bone regeneration in a
School of Medicine (No. 2015-0083) and conformed to canine cranial model using allogeneic adipose derived stem cells and
the principles outlined in the Declaration of Helsinki. coral scaffold. Biomaterials 2013;34:2655-64.

Plastic and Aesthetic Research ¦ Volume 3 ¦ October 25, 2016 327