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and kept refrigerated. The skin is measured and released from 42.2% of donors had to be discarded secondary to
after the third negative microbiological results. contamination, and 6.7% of tissues were partially discarded.
At the end of the period, tissue from one donor (2 batches)
The batch is discarded should there be positive culture was still under quarantine [Table 2]. Eighty‑four point four
results with any germs in any of the phases. The discarded percent of retrievals of skin were from the back and lower
skin is not counted, as measurements are carried out only limbs, as specified in Table 2.
following the final phase.
The total amount of collected skin tissue during the period
Cryopreservation was not used as the skin was stored of evaluation was 31,314.63 cm². The largest amount of
in a refrigerator at 4 °C ± 2 °C. The skin is maintained retrieved tissue from a single donor totaled 2,453.6 cm² and
in a 90% sterile glycerol solution prior to packaging. All the lowest amount was 422.2 cm². The obtained average
allografts were nonmeshed. amount was 1,252.59 cm² per donor, as shown in Table 3.

RESULTS Tissues retrieved from the 45 donors generated 81 processing
batches in the 1st year of operation; 41 were of acceptable
quality for clinical use, 38 were discarded due to an
In its 1st year of operation, the skin bank of the unacceptable biological burden, and 2 remained in
HUEC retrieved skin tissue from 45 cadaveric donors quarantine at the end of the period. Thirty‑eight of
(46.6% female and 53.3% male) with an average age of the batches released for use were transplanted and 3
36.4 years (range: 15‑60 years).
remained in storage [Table 3].
Most donors were Caucasian (91%); there were no donations Fourty‑two point one percent of tissue discard was due to
from black or yellow skin‑colored donors. The most frequent detection of coagulase‑negative staphylococci. Two batches
cause of death was of neurological origin, with traumatic from a single donor were positive for more than one
brain injury and stroke accounting for 55.6% of cases. contaminant. Reasons and contaminants of discarded
Data related to gender, age, race and cause of death is tissues are specified in Table 3.
presented in Table 1 below. As observed in Table 4, tissues from a single donor

All skin tissue retrievals took place in hospitals within benefited up to 5 patients. A total of 30 patients received
Curitiba city, with 44% in the HUEC, followed by other tissue during this period.
major trauma hospitals [Table 2].
Table 2: Skin tissue retrieval
Regarding the use of donated skin, tissue from 48.9%
of donors was released for clinical application. Tissue Variable Descriptive
statistics [n (%)]
Institution
Table 1: Skin donors profile
Evangelical University Hospital of Curitiba 20 (44.44)
Variable Descriptive statistics n (%) Cajuru University Hospital 8 (17.78)
Gender Workers×Hospital 6 (13.33)
Female 21 (46.67) São Vicente Hospital 3 (6.67)
Male 24 (53.33) Vitória Hospital 1 (2.22)
Total 45 (100.00) Angelina Caron Hospital 1 (2.22)
Age (in years) Pilar Hospital 1 (2.22)
Average ( ± SD) 36.42 (5) Nations’ Hospital 1 (2.22)
Maximum 60 Zilda Arns Hospital for Elderly 1 (2.22)
Minimum 15 Red Cross Hospital 1 (2.22)
Skin color UFPR* Clinical Hospital 1 (2.22)
White 41 (91.11) Vita Batel Hospital 1 (2.22)
Brown 4 (8.89) Total 45 (100.00)
Cause of death Donor areas
Severe TBI 13 (28.89) Back and legs (anterior and posterior) 38 (84.44)
Stroke 12 (26.67) Lower limbs (anterior and posterior) 5 (11.11)
Polytrauma 7 (15.56) Abdomen and legs (anterior and posterior) 2 (4.44)
CHF*/respiratory insufficiency 3 (6.67) Retrieved tissue outcomes (number of donors)
WGF † 3 (6.67) All tissues acceptable for use 22 (48.89)
Preeclampsia 1 (2.22) All tissues discarded 19 (42.22)
NW ‡ 1 (2.22) Partial discard 3 (6.67)
Lung cancer 1 (2.22) Skin still being processed 1 (2.22)
2
HÁ § 1 (2.22) Areas (in cm ) of retrieved skins tissue
Chronic pancreatitis 1 (2.22) Total 31,314.63
Suicide 1 (2.22) Maximum per donor 2,453.60
Dissecting aneurysm 1 (2.22) Minimum per donor 422.20
Average per donor ( ± SD) 1,252.59 (530.38)
*Congestive heart failure, Wound by gunfire, Knife wound, Heart attack,
‡
†
§
SD: Standard deviation, TBI: Traumatic brain injury *The Federal University of Paraná, SD: Standard deviation
328 Plast Aesthet Res || Vol 2 || Issue 6 || Nov 12, 2015