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Amatori et al. RT-qPCR array for gene expression

first observation made in HeLa cells, could be put in Conflicts of interest
relation with the known role of these CDKs in apoptosis, There are no conflicts of interest.
in addition to their known functions in regulation of cell
cycle progression. This hypothesis is supported by Patient consent
[20]
evidence that CDK2 silencing by shRNA decreases Not applicable.
cisplatin-induced apoptosis and PARP cleavage in
embryonal carcinoma models and opens the door Ethics approval
[21]
to possible future insights. Not applicable.
Regarding the effects of NaBu, our data confirm REFERENCES
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Authors’ contributions 13. Guerzoni C, Amatori S, Giorgi L, Manara MC, Landuzzi L, Lollini
PL, Tassini A, Balducci M, Manfrini M, Pratelli L, Serra M, Picci
Study conception and design: M. Fanelli, S. Amatori P, Magnani M, Fusi V, Fanelli M, Scotlandi K. An aza-macrocycle
Primers design and PCR conditions optimization: S. containing maltolic side-arms (maltonis) as potential drug against
Amatori human pediatric sarcomas. BMC Cancer 2014;14:137.
Validation of the RT-qPCR array: G. Persico 14. Rippo MR, Villanova F, TomassoniArdori F, Graciotti L, Amatori S,
Manuscript writing and editing: M. Fanelli, S. Amatori Manzotti S, Fanelli M, Gigante A, Procopio A. Dexamethasone affects
Fas- and serum deprivation-induced cell death of human osteoblastic
Financial support and sponsorship cells through survivin regulation. Int J Immunopathol Pharmacol
2010;23:1153-65.
This work was supported by Associazione a Sostegno 15. Andersen CL, Jensen JL, Ørntoft TF. Normalization of real-time
degli Studi Oncologici (ASSO), Fano (PU), Italy. quantitative RT-PCR data: a model based variance estimation
98 Journal of Cancer Metastasis and Treatment ¦ Volume 3 ¦ May 24, 2017