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Frame et al. Investigating models for prostate cancer research

as being more radiation-resistant . One report the colony forming ability of selected subpopulations
[15]
showed that stem-like cells from the Du145 cell line of primary prostate epithelial cells, including stem-like
were more resistant to etoposide . However, there cells and TA cells, were analyzed following treatment
[21]
is currently no experimental evidence determining with etoposide [Figure 3A]. Cancer stem-like cells
the effect of chemotherapeutic agents specifically on showed increased ability to form colonies compared
primary prostate cancer stem-like cells. Therefore, to TA cells post-treatment. Since etoposide is known to

Figure 2: A combination of radiation treatment and Vorinostat has varied effects on viability and colony forming ability in a panel of cell
lines and primary prostate epithelial cells. PNT1a, BPH-1, P4E6 and PC3 cells were treated with Vorinostat (10 µmol/L) or radiation (75
Gy) or both and measured using alamar blue assay at 24 h (A), 48 h (B) and 72 h (C) post-treatment. Primary epithelial cultures from six
patients, normal (2 samples) (D) and prostate cancer (4 samples) (E) were treated with Vorinostat (10 µmol/L) or radiation (75 Gy) or both
and measured using alamar blue assay at 24 h post-treatment. Each symbol represents a different patient sample; (F) primary epithelial
cultures from four patients were treated with 2 Gy radiation or three concentrations of Vorinostat (low: 0.625 µmol/L, medium: 2.5 µmol/L,
high: 10 µmol/L) or both and assessed for colony forming ability 10-14 days after growth. Colony forming ability is presented as % surviving
fraction
306 Journal of Cancer Metastasis and Treatment ¦ Volume 3 ¦ December 6, 2017