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Frame et al. Investigating models for prostate cancer research

to show any differential response of the combination to postoperative radiotherapy in prostate cancer .
[36]
treatment would be required. This could be something Alternatively, a pre-treatment prognostic gene
similar to the PORTOS score; predictor of response signature could be of use to decide which patients

Figure 5: Signatures of two populations of cells within primary prostate cultures can be characterized from quantitative phase imaging (QPI)
data and used to identify different cell populations within heterogeneous cultures. (A) Immunofluorescence of CD49b in a mixed culture of
cells, transit amplifying (TA) cells and committed basal (CB) cells; (B) flow cytometry of TA and CB cells using the CD49b surface marker; (C)
QPI images of TA and CB cells showing cell segmentation outlines (colored lines). Data from QPI analysis of each cell type was measured
including (D) mean cell dry mass, (E) mean cell area and (F) cell sphericity; (G) analysis of a mixed culture of cells with gates applied to
separate out the two cell populations on the basis of cell area. Data from the whole population (WP) and each cell type was measured and
plotted as mean cell area and mean cell dry mass
310 Journal of Cancer Metastasis and Treatment ¦ Volume 3 ¦ December 6, 2017