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demonstrated and they are often found effective in hepatic 10. Vieira TO, Said A, Aboutabl E, Azzam M, Creczynski-Pasa TB.
disorders. [40-42] Previous studies have reported that Bombax Antioxidant activity of methanolic extract of Bombax ceiba. Redox
Rep 2009;14:41-6.
ceiba extract possesses in vitro antioxidant activity. [10-12] 11. Gandhare B, Soni N, Dhongade HJ. In vitro antioxidant activity of
Based on this, it can be hypothesized that the observed Bombax ceiba. Int J Biomed Res 2010;1:31-6.
hepatoprotection offered by BCAE may be ascribed to its 12. Jain V, Verma SK, Katewa SS, Anandjiwala S, Singh B. Free radical
antioxidant activity. Furthermore, this was supported by scavenging property of Bombax ceiba Linn. root. Res J Med Plant
the observation that daily treatment with silymarin, a well 2011;5:462-70.
proven antioxidant, showed similar effects on CCl -induced 13. You YJ, Nam NH, Kim Y, Bae KH, Ahn BZ. Antiangiogenic activity
of lupeol from Bombax ceiba. Phytother Res 2003;17:341-4.
4
changes in the levels of hepatic function markers and 14. Ravi V, Patel SS, Verma NK, Datta D, Saleem TSM. Hepatoprotective
similarly prevented the CCl -induced damage to the liver. activity of Bombax ceiba Linn against isoniazid and rifampicin-
4
The in vivo antioxidant activity and hepatoprotective effect induced toxicity in experimental rats. Int J Appl Res Nat Prod
of silymarin are well demonstrated in earlier studies [21,22] 2010;3:19-26.
and corroborate with the present findings. The observed 15. Wong SP, Leong LP, Koh JHW. Antioxidant activities of aqueous
extracts of selected plants. Food Chemistry 2006;99:775-83.
hepatoprotective effect of BCAE was comparable to that 16. Khandelwal KR. Practical pharmacognosy. 15th ed. Pune: Nirali
of silymarin. Prakashan; 2006. p. 149.
17. Organization for Economic Cooperation and Development (OEDC),
In conclusion, BCAE exhibited protective effect on Guideline for the testing of chemicals. Revised draft guideline 423.
CCl -induced free radical mediated hepatotoxicity. The Document on acute oral toxicity and acute toxicity class method;
4
2001. Available from: www.oecd.org.
observed hepatoprotection by BCAE may be a consequence 18. Sengottuvelu S, Duraisami S, Nandhakumar J, Duraisami R,
of its antioxidant effect due to the presence of flavonoids Vasudevan M. Hepatoprotective activity of Camellia sinensis and its
or other phenolic compounds in BCAE. The present possible mechanism of action. Iranian J Pharmacol Ther 2008;7:9-14.
investigations scientifically validate the traditional use of 19. Ye X, Feng Y, Tong Y, Ng KM, Tsao S, Lau GK, Sze C, Zhang Y,
flowers of Bombax ceiba in hepatic disorders. Tang J, Shen J, Kobayashi S. Hepatoprotective effects of Coptidis
rhizoma aqueous extract on carbon tetrachloride-induced acute liver
hepatotoxicity in rats. J Ethnopharmacol 2009;124:130-6.
Acknowledgments 20. Sorg DA, Buckner B. A simple method of obtaining venous blood from
Authors are thankful to Dr. Vinay Lomesh, Shree Ram small laboratory animals. Proc Soc Exp Biol Med 1964;115:1131-2.
Institute of Industrial Research, New Delhi, for his help in 21. Bindoli A, Cavallini L, Siliprandi N. Inhibitory action of silymarin on
interpretation of histological changes in liver tissues. lipid peroxide formation in rat liver mitochondria and microsomes.
Biochem Pharmacol 1977;26:2405-9.
22. Lettéron P, Labbe G, Degott C, Berson A, Fromenty B, Delaforge
Financial support and sponsorship M, Larrey D, Pessayre D. Mechanism for the protective effects of
Nil. silymarin against carbon tetrachloride-induced lipid peroxidation
and hepatotoxicity in mice. Evidence that silymarin acts both as an
Conflicts of interest inhibitor of metabolic activation and as a chain-breaking antioxidant.
There are no conflicts of interest. Biochem Pharmacol 1990;39:2027-34.
23. Reitman S, Frankel S. A colorimetric method for determination of
serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J
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