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Original Article

Aqueous extract of
Aqueous extract of Corchorus olitoriusCorchorus olitorius decreases cytotoxicity decreases cytotoxicity
of afl atoxin Bfl atoxin B and fumonisin B and fumonisin B in H4IIE- in H4IIE-lucluc cells cells
of a
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Mohamed I. M. Ibrahim , Rialet Pieters , Sekena H. Abdel-Aziem , Anna M. van der Walt ,
2
1
3
2
Cornelius C. Bezuidenhout , John P. Giesy 4,5,6,7,8,9 , Mosaad A. Abdel-Wahhab 1
2
1 Department of Food Toxicology and Contaminants, National Research Centre, Dokki, Cairo 12622, Egypt
2 Unit of Environmental Sciences and Management, North-West University, Private Bag X6001, Potchefstroom 2520, South Africa
3 Department of Cell Biology, National Research Centre, Dokki, Cairo 12622, Egypt
4 Department of Veterinary Biomedical Sciences and Toxicology Centre, University of Saskatchewan, Saskatoon,
Saskatchewan S7N 5B4, Canada
5 Department of Zoology, and Center for Integrative Toxicology, Michigan State University, East Lansing, MI 48824, USA
6 Department of Biology and Chemistry and State Key Laboratory in Marine Pollution, City University of Hong Kong, Kowloon,
Hong Kong, China
7 School of Biological Sciences, University of Hong Kong, Hong Kong, China
8 State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093,
Jiangsu, China
9 Department of Biology, Hong Kong Baptist University, Hong Kong, China

ABSTRACT
Aim: Afl atoxin B (AFB ) and fumonisin B (FB ) are important food-borne mycotoxins. Co-contamination of foodstuffs with
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these two mycotoxins is well-known and has been implicated in a possible development of hepatocellular carcinoma in
humans living in regions of the world where exposures to these mycotoxins in grain are greatest. The aim of the current study
was to evaluate the potential protective effects of an aqueous extract of Cochorus olitorius (C. olitorius, moroheiya) against
cytotoxicity of AFB and/or FB in H4IIE-luc rat hepatoma cells, using assays to measure cell viability and disruption of DNA
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integrity. Although this transactivation assay was originally developed to specifi cally respond to aryl hydrocarbon agonists,
this cell line was used because of its hepatic origin. Methods: H4IIE-luc cells were incubated with different concentrations
of AFB and/or FB for 24 and 48 h with or without aqueous extract of C. olitorius. Results: Both mycotoxins decreased cell
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viability and increased DNA damage. Cytotoxicity was more pronounced when cells were exposed simultaneously to AFB
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and FB . Conclusion: Aqueous extract of C. olitorius protected cells against cytotoxicity of mycotoxins. C. olitorius contains
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a water-soluble, natural chemo-preventative agent for cancer that should be isolated and identifi ed.
Key words: Anticancer; cytotoxicity; DNA; liver; moroheiya; mycotoxins

Address for correspondence:
Prof. Mosaad A. Abdel-Wahhab, Department of Food Toxicology and Contaminants, National Research Centre, Dokki, Cairo 12622, Egypt.
E-mail: mosaad_abdelwahhab@yahoo.com
Received: 11-01-2015, Accepted: 27-03-2015

INTRODUCTION
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Website: Co-occurrence of various mycotoxins in foodstuffs and
http://www.hrjournal.net/ animal feed is common because each toxigenic fungus can
produce more than one mycotoxin and foodstuff can be
DOI: colonized by several fungi either while growing in the field
[1]
10.4103/2394-5079.155692 or during storage or transport. Processed products are
often composed of various raw materials which might be

Hepatoma Research | Volume 1 | Issue 2 | July 15, 2015 75