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C. olitorius 20 μg/mL C. olitorius 40 μg/mL
AFB 1 24 h AFB 1 48 h C. olitorius 20 μg/mL C. olitorius 40 μg/mL
FB 1 48 h
AFB 1 + C.olitorius 20 μg/mL AFB 1 + C.olitorius 40 μg/mL FB 1 24 h
FB 1 + C. olitorius 20 μg/mL FB 1 + C. olitorius 40 μg/mL
120 **
105
100
110
95
***
100 90
85
90 80
** * *
75 *
80
70
* * **
65
70 **
* 60
55
60
% Cell viability 50 *** % Cell viability 50
45
40 *** 40
35
30
30
25
***
20 20
*** 15
10 *** 10
*** *** 5
*** ***
0 0
0.025 0.250 2.500 25.000 50.000 0.1 1.0 10.0 100.0 200.0
a FB 1 concentration (μmol/L)
AFB 1 concentraion (μmol/L) b
C. olitorius 20 μg/mL C. olitorius 40 μg/mL
FB 1 + AFB 1 24 h FB 1 + AFB 1 48 h
(FB 1 + AFB 1 ) + C. olitorius 20 μg/mL (FB 1 + AFB 1 ) + C. olitorius 40 μg/mL
115
110
105
100
95
90 *
85
80
75
70 *** ***
% Cell viability 60 *** ***
65
55
50
45
40
35 ***
***
30
25 ***
20 *** ***
15
10 ***
5 ***
***
0 ***
-5
0.125 1.250 12.500 125.000 250.000
AFB 1 + FB 1 concentrations (μmol/L)
c
Figure 1: Cytotoxcity of (a) AFB at concentrations of 0.25-50 μmol/L without and with C. olitorius extract, (b) FB at concentration of 1-200 μmol/L without and
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1
with C. olitorius extract, and (c) AFB at concentrations of 0.25-50 μmol/L together with concentrations of 1-200 μmol/L FB , on proliferation of H4IIE-luc cell
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line determined by MTT bioassay. Data represent mean ± SEM of triplicates (signifi cance of the differences among treatment groups: *P < 0.05; **P < 0.01;
***P < 0.001). AFB : afl atoxin B ; FB : fumonisin B ; C. olitorius: Cochorus olitorius; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide;
1
1
1
1
SEM: standard error mean
Hepatoma Research | Volume 1 | Issue 2 | July 15, 2015 79