Page 42 - Read Online

Folic
Beta-carotene Myristic
Arachidic
Behenic
Total carotenoids Lignoceric
Stearic
Palmitoleic
Total phenolics Palmitic
Linoleic
Linolenic
0 500 1000 1500 2000 2500
a b 0 200 400 600 800 1000 1200 1400
mg/100 g dry mass mg/100 g dry mass

c d
Figure 1: (a) Total phenolics, total carotenoids, and β-carotene content; (b) fatty acid profi les and folic acid content; (c) calcium and magnesium concentration; and (d)
trace elements (iron, zinc, and selenium) concentration of Amaranthus hybridus extract

a b

c d
Figure 2: The cytotoxic effects of AFB at different concentrations (μmol/L) without/with Amaranthus hybridus extract: (a) FB at different concentrations (μmol/L)
1 1
without/with Amaranthus hybridus; (b) AFB plus with FB1without/with Amaranthus hybridus; (c) Amaranthus hybridus extract only; and (d) on proliferation of H4IIE-luc
1
cell line determined by MTT bioassay. FB : fumonisin B ; AFB : afl atoxin B ; MTT: methyl thiazol tetrazolium
1 1 1 1
EC values for AFB were 10.5 and 1.8 μmol/L after 24 and addition of 20 or 40 μg/mL A. hybridus extract, respectively.
50
1
48 h of exposure, respectively. When the plant extract was No discernible cytotoxicity was observed in cells, based
added at 20 and 40 μg/mL, the EC values were 3.88 and 7.67 on the MTT assay, when cells were exposed to FB at
1
50
μmol/L after 48 h of exposure, respectively. On the other lesser concentrations. Whereas, at greater concentrations
hand, the EC for the combined mycotoxins (AFB + FB ) sufficient cell mortality was exhibited in the MTT assay
1
1
50
was 24.02 and 5.86 μmol/L after 24 and 48 h, respectively. that indicates cytotoxicity at dosages of 200 μmol/L but
While the 50% inhibition of proliferation for AFB plus FB the quantities tested were still substantially less than those
1
1
with plant extract were 7.30 and 14.0 μmol/L after the required to obtain EC .
50
140 Hepatoma Research | Volume 1 | Issue 3 | October 15, 2015