Page 38 - Read Online
P. 38
Topic: Natural Products and Hepatocellular Carcinoma
Protective effects of
Protective effects of Amaranthus hybridusAmaranthus hybridus against afl atoxin B against afl atoxin B
1
and fumonisin Band fumonisin B -induced genotoxicity in H4IIE--induced genotoxicity in H4IIE-lucluc cells cells
1
Mohamed I. M. Ibrahim , Rialet Pieters , Sekena H. Abdel-Aziem , Anna M. van der Walt ,
2
2
3
1
Cornelius C. Bezuidenhout , John P. Giesy 4,5,6,7,8,9 , Mosaad A. Abdel-Wahhab 1
2
1 Food Toxicology and Contaminants Department, National Research Center, Dokki, Cairo 12622, Egypt
2 Unit of Environmental Sciences and Management, North-West University, Private Bag X6001, Potchefstroom 2520, South Africa
3 Cell Biology Department, National Research Center, Dokki, Cairo 12622, Egypt
4 Department of Veterinary Biomedical Sciences and Toxicology Centre, University of Saskatchewan, Saskatoon,
Saskatchewan S7N 5B4, Canada
5 Department of Zoology, and Center for Integrative Toxicology, Michigan State University, East Lansing, MI 48824, USA
6 Department of Biology and Chemistry and State Key Laboratory in Marine Pollution, City University of Hong Kong, Kowloon,
Hong Kong, China
7 School of Biological Sciences, University of Hong Kong, Hong Kong, China
8 State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093,
Jiangsu, China
9 Department of Biology, Hong Kong Baptist University, Hong Kong, China
ABSTRACT
Aim: Protective effects of aqueous extract of Amaranthus hybridus against afl atoxin B (AFB ) and/or fumonisin B (FB ) on
1 1 1 1
the H4IIE-luc cell line were determined by use of the methyl thiazol tetrazolium viability assay and disruption of DNA integrity.
Methods: H4IIE-luc cells were incubated with different concentrations of AFB and/or FB for 24 and 48 h with or without aqueous
1 1
extract of A. hybridus. Results: AFB decreased the viability of cells after 24 and 48 h of exposure. EC values for AFB were
1 50 1
10.5 and 1.8 μmol/L for the two periods, respectively. When the 48 h exposure to mycotoxin repeated with a pre-treatment
of 20 and 40 μg/mL extract of A. hybridus, the EC changed to 3.88 and 7.67 μmol/L, respectively. H4IIE-luc cells exposed
50
to FB for 24 h responded more than those incubated for 48 h. Cells treated with a combination of AFB and FB were less
1 1 1
viable with a signifi cant decrease in the greater concentration. The mixture of AFB and FB resulted in a signifi cant threat to
1 1
H4IIE-luc as indicated by the absence or appearance of new bands in random amplifi ed polymorphic DNA analysis, which
demonstrated damage to DNA. The protective effects were probably due to greater content of total phenolics, carotenoids,
β-carotene, folic-, linolenic-, linoleic and palmitic acids, as well as calcium, magnesium, iron, zinc, and selenium observed in the
extract. Conclusion: Exposure to 40 μg/mL of extract of A. hybridus protected cells from damage to DNA by stabilizing DNA.
Key words: Afl atoxin B ; Amaranthus hybridus; cytotoxicity; DNA; fumonisin B ; hepatoma cells; methyl thiazol tetrazolium assay
1 1
Address for correspondence:
Prof. Mosaad A. Abdel-Wahhab, Food Toxicology and Contaminants Department, National Research Center, Dokki, Cairo 12622, Egypt.
E-mail: mosaad_abdelwahhab@yahoo.com
Received: 19-07-2015, Accepted: 23-09-2015
This is an open access article distributed under the terms of the Creative
Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows
Access this article online others to remix, tweak, and build upon the work non-commercially, as long as the
Quick Response Code author is credited and the new creations are licensed under the identical terms.
Website:
http://www.hrjournal.net/ For reprints contact: reprints@medknow.com
How to cite this article: Ibrahim MI, Pieters R, Abdel-Aziem SH,
DOI: van der Walt AM, Bezuidenhout CC, Giesy JP, Abdel-Wahhab MA.
10.4103/2394-5079.167377 Protective effect of Amaranthus hybridus against afl atoxin B and fumonisin
1
B -induced genotoxicity in H4IIE-luc cells. Hepatoma Res 2015;1:136-46.
1
136 © 2015 Hepatoma Research | Published by Wolters Kluwer - Medknow