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Page 12 of 17       Cabral et al. Microstructures 2023;3:2023040  https://dx.doi.org/10.20517/microstructures.2023.39

               detector”. Conversely, it allows the selection of a region of interest in the image to produce a Bragg Vector
               Map (BVM), which collapses the diffraction information into a single image [100,101] . This information can be
               collected at various points in the image for further analysis, such as orientation mapping and strain
               mapping. Furthermore, py4DSTEM has built-in functionalities that enable users to locate diffraction disks
               and make quantitative measurements for parameters such as strain and polarization. This capability ensures
               that measurements can be standardized and repeated across a variety of datasets, facilitating repeatable
               analysis.


               Although programs, such as py4DSTEM, provide a strong foundation for analyzing 4D-STEM datasets,
               there are cases where researchers need to develop custom tools for their specific applications. Automated
               analysis is the most practical approach for such analyses, but often, custom solutions need to be built. For
               instance, some datasets may contain noisy and complex features that require filtering and fitting algorithms.
               One recently introduced program is AutoDisk, a Python-based code that performs automated diffraction
               processing for strain mapping. Variations in diffraction patterns can arise due to various factors, including
               thickness gradients and low probe currents for beam-sensitive materials, which can complicate automated
               analysis. AutoDisk addresses these variations by utilizing cross-correlations, blob detection, edge
               refinements, and lattice fitting to identify diffraction disks . Once identified, this diffraction information
                                                                 [102]
               can be used for various analyses, including characterizing phase, symmetry, and orientation. While there are
               many ways to analyze data, unique solutions may be necessary for analyzing specific datasets. There are
               numerous code repositories available for 4D-STEM data analysis, including py4DSTEM, HyperSpy, pyXem,
                                                                             [19]
               LiberTEM, and Pycroscopy, which can serve as a basis for custom analysis .

               SUMMARY AND OUTLOOK
               In conclusion, electron microscopy is a dynamic and continuously advancing technique with immense
               potential for the analysis of ferroic and other functional materials. Ferroic materials exhibit a wide range of
               unique properties that can be utilized in countless applications. These properties stem from chemical and
               structural inhomogeneities that occur at the atomic scale.


               S/TEM offers a distinct advantage in probing these features in both real space and reciprocal space through
               electron diffraction measurements. Recent advancements in electron microscopy technology have improved
               the usability and enabled unprecedented resolution of these instruments. To fully harness the potential of S/
               TEM in the development of advanced materials, it is crucial to make data collection and analysis widely
               accessible to researchers. This accessibility will foster further exploration and utilization of S/TEM in
               material characterization. The field holds incredible potential, which can be further realized through
               ongoing advancements and the collaboration of researchers from various disciplines.


               (1) Instrumentation availability and data analysis tools: STEM and TEM play a crucial role in the study of
               piezoelectric and other functional materials. While state-of-the-art instruments are not immediately
               available to all researchers, instruments are often accessible to external researchers at universities, national
               laboratories, and in industry. There are two aspects to make TEM and STEM available to more researchers:

               a. Data collection: Modern S/TEM user interfaces are equipped with programmatic capabilities, enabling
               users to develop workflows to streamline data collection. S/TEMs can readily interface with Python code or
               support the user of custom scripts such as Gatan’s Digital Micrograph. It is essential to promote the open-
               source nature of these programs so they can be utilized by researchers from various backgrounds.
               Simplifying data collection will allow researchers to allocate more time for analysis and characterization.
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