Page 75 - Read Online
P. 75
a double centrifugation technique. Regardless of the REFERENCES
[12]
rate of centrifugation or the time of centrifugation, a
single spin cannot adequately concentrate platelets, 1. Mekkes JR, Loots MA, Van Der Wal AC, Bos JD. Causes, investigation and
because the red blood cells will interfere with their fine treatment of leg ulceration. Br J Dermatol 2003;148:388‑401.
separation. [4] 2. Anderson I. Aetiology, assessment and management of leg ulcers. Wound
Essent 2006;1:20‑36.
3. Braund R, Hook S, Medlicott NJ. The role of topical growth factors in chronic
A study conducted by Frykberg et al. on 49 patients wounds. Curr Drug Deliv 2007;4:195‑204.
[13]
with 65 nonhealing ulcers showed that 63 of 65 4. Bhanot S, Alex JC. Current applications of platelet gels in facial plastic surgery.
ulcers responded with a reduction in area, volume Facial Plast Surg 2002;18:27‑33.
and undermining of the ulcers in a mean duration of 5. Vaishnavi C, Mohan B, Narayanan LL. Treatment of endodontically
2.8 weeks with 3.2 treatments. induced periapical lesions using hydroxyapatite, platelet‑rich plasma, and a
combination of both: an in vivo study. J Conserv Dent 2011;14:140‑6.
6. Sarvajnamurthy S, Suryanarayan S, Budamakuntala L, Suresh DH. Autologous
[14]
Steenvoorde et al. conducted a study on 12 patients platelet rich plasma in chronic venous ulcers: study of 17 cases. J Cutan Aesthet
with 13 wounds, showing that 7 of 13 wounds Surg 2013;6:97‑9.
required more than 1 application, with a mean number 7. Blume PA, Walters J, Payne W, Ayala J, Lantis J. Comparison of negative
of 2.2 applications and a mean treatment period of pressure wound therapy using vacuum‑assisted closure with advanced
moist wound therapy in the treatment of diabetic foot ulcers: a multicenter
4.2 weeks. randomized controlled trial. Diabetes Care 2008;31:631‑6.
8. Yuan T, Zhang CQ, Tang MJ, Guo SC, Zeng BF. Autologous platelet rich
Kakudo et al. treated five cases of intractable skin ulcer plasma enhances wound healing in chronic wounds. Wounds 2009;21:280‑5.
[15]
with autologous PRP, among which three ulcers healed 9. Knighton DR, Ciresi KF, Fiegel VD, Austin LL, Butler EL. Classification and
completely within 4 weeks and epithelization of wound treatment of chronic nonhealing wounds. Successful treatment with autologous
platelet‑derived wound healing factors (PDWHF). Ann Surg 1986;204:322‑30.
occurred within 6.6 weeks on average. 10. Kakudo N, Kushida S, Minakata T, Suzuki K, Kusumoto K. Platelet‑rich
plasma promotes epithelialization and angiogenesis in a splitthickness skin
In our study, 24 patients with 33 ulcers were treated graft donor site. Med Mol Morphol 2011;44:233‑6.
with PRP and the mean duration of healing of the ulcers 11. Sánchez AR, Sheridan PJ, Kupp LI. Is platelet‑rich plasma the perfect
was 5.6 weeks (SD 3.23). We also noticed a decrease in enhancement factor? A current review. Int J Oral Maxillofac Implants
pain. 2003;18:93‑103.
12. Marx RE. Platelet‑rich plasma (PRP): what is PRP and what is not PRP? Implant
Dent 2001;10:225‑8.
Chronic wounds are a frequent problem in developing 13. Frykberg RG, Driver VR, Carman D, Lucero B, Borris‑Hale C, Fylling CP,
countries. Because these wounds lack the necessary Rappl LM, Clausen PA. Chronic wounds treated with a physiologically relevant
GFs for healing, they are often difficult to heal and are concentration of platelet‑rich plasma gel: a prospective case series. Ostomy
frequently complicated by superinfection. PRP contains Wound Manage 2010;56:36‑44.
various GFs that are necessary in wound healing. In 14. Steenvoorde P, van Doorn LP, Naves C, Oskam J. Use of autologous platelet‑
rich fibrin on hard‑to‑heal wounds. J Wound Care 2008;17:60‑3.
addition, the high concentration of leukocytes present in 15. Kakudo N, Kushida S, Ogura N, Hara T, Suzuki K. The use of autologous
PRP is also helpful in preventing infections. [16] platelet rich plasma in the treatment of intractable skin ulcer. Open J Reg
Med 2012;1:29‑32.
Currently, there is a paucity of critical scientific data 16. Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, Gogly B.
regarding the beneficial effects of PRP in clinical Platelet‑rich fibrin (PRF): a second‑generation platelet concentrate. Part III:
procedures. In the current study, PRP was found to leucocyte activation: a new feature for platelet concentrates? Oral Surg Oral
Med Oral Pathol Oral Radiol Endod 2006;101:e51‑5.
be useful in treating chronic leg ulcers. However,
further controlled, randomized prospective clinical How to cite this article: Suryanarayan S, Budamakuntla L, Khadri SI,
trials are necessary to definitively demonstrate its Sarvajnamurthy S. Efficacy of autologous platelet-rich plasma in
efficacy. There is also a need for the development the treatment of chronic nonhealing leg ulcers. Plast Aesthet Res
2014;1:65-9.
of a standard protocol for the preparation of PRP, as
literature currently there is no standardization of the Source of Support: Nil, Conflict of Interest: None declared.
procedure. Received: 01-04-2014; Accepted: 10-07-2014
Plast Aesthet Res || Vol 1 || Issue 2 || Sep 2014 69