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of microglia, which act as sentinels of the CNS. Normal   13.  Paolicelli RC, Bolasco G, Pagani F, Maggi L, Scianni M, Panzanelli P,
           HE staining and specialised silver-gold staining of brain   Giustetto M, Ferreira TA, Guiducci E, Dumas L, Ragozzino D, Gross CT.
           tissue show morphologic variations of these cells in   Synaptic pruning by microglia is necessary for normal brain development.
                                                                  Science 2011;333:1456-8.
           both pre and postnatal stages of the developing rat brain,   14.  Tremblay ME, Lowery RL, Majewska AK. Microglial interactions with
           which are further supported with an ultrastructural,   synapses are modulated by visual experience. PLoS Biol 2010;8:e1000527.
           SEM study. The microglial identity of the isolated cells   15.  Tremblay ME, Majewska AK. A role for microglia in synaptic plasticity?
                                                                  Commun Integr Biol 2011;4:220-2.
           was confirmed with a CD11b marker. The cells in the   16.  Wake H, Moorhouse AJ, Jinno S, Kohsaka S, Nabekura J. Resting
           normally developing brain also show an age dependent   microglia directly monitor the functional state of synapses in vivo and
           variation in their baseline phagocytic capacity, which is   determine the fate of ischemic terminals. J Neurosci 2009;29:3974-80.
           higher in the perinatal phase and assumed to be related   17.  Hanisch UK, Kettenmann H. Microglia: active sensor and versatile effector
                                                                  cells in the normal and pathologic brain. Nat Neurosci 2007;10:1387-94.
           with the normal physiology of CNS development and   18.  Kim SU, de Vellis J. Microglia in health and disease.  J Neurosci Res
           function. Therefore, changes in age sensitive microglial   2005;81:302-13.
           morphology and phagocytic activity from the embryonic   19.  Block  ML,  Zecca  L,  Hong  JS.  Microglia-mediated  neurotoxicity:
                                                                  uncovering the molecular mechanisms. Nat Rev Neurosci 2007;8:57-69.
           to the adult brain could be an important reference when   20.  Kreutzberg GW. Microglia: a sensor for pathological events in the CNS.
           considering any neuropathological conditions with      Trends Neurosci 1996;19:312-8.
           microglial involvement.                             21.  Ghosh A, Mukherjee J, Bhattacharjee M, Sarkar P, Acharya S, Chaudhuri
                                                                  S and Chaudhuri S. The Other Side of the Coin: Beneficiary Effect of
                                                                  ‘Oxidative Burst’ Upsurge with T11TS Facilitates the Elimination of
           Acknowledgments                                        Glioma Cells. Cell Mol Biol 2007;53:53-62.
           The authors are grateful to Dr. Srikanta Chakroborty of   22.  Ochiogu IS, Uchendu CN, Ihedioha JI. A new and simple method of
           the USIC, Burdwan University, WB, India for providing   confirmatory detection of mating in Albino rats (Rattus norvegicus).
                                                                  Animal Research International 2006;3:527-30.
           technical support for SEM.                          23.  Penfield W, Cone W. Neuroglia and microglia (the metallic methods). In:
                                                                  McClung CE, editer. In Handbook of Microscopical Technique. New York:
           Financial support and sponsorship                      Paul B Hoeber Inc.; 1937.p. 489-521.
           The work is financially supported by UGC-MRP Grant   24.  McCarter JC. A Silver Carbonate Staining method for oligodendrocytes
                                                                  and microglia for routine use. Am J Pathol 1940;16:233-235.2.
           [No. F. PSW-169/09-10 (ERO)] and by DST- YS (SERB)   25.  Begum Z, Sarkar S, Mukherjee J, Ghosh A, Chaudhuri S, Chaudhuri
           Project Grant [No.- SR/FT/LS-81/2011], Govt. of India.  S. Evaluation of anti-tumor property of specific and nonspecific BRMS
                                                                  in experimental glioma by assessing the microglial cell functional and
           Conflicts of interest                                  phenotypic modulations. Cancer Biol Ther 2003;2:356-63.
           There are no conflicts of interest.                 26.  Ford AL, Goodsall AL, Hickey WF, Sedgwick JD. Normal adult ramified
                                                                  microglia separated from other central nervous system macrophages by
                                                                  flow cytometric sorting. Phenotypic differences defined and direct  ex
           REFERENCES                                             vivo antigen presentation to myelin basic protein-reactive CD4+ T cells
                                                                  compared. J Immunol 1995;154:4309-21.
           1.   Ginhoux F, Lim S, Hoeffel G, Low D, Huber T. Origin and differentiation   27.  Chen X, Zhang Y, Sadadcharam G, Cui W, Wang JH. Isolation,
              of microglia. Front Cell Neurosci 2013;7:1-14.      purification, and culture of primary murine microglia cells. Bio-protocol
           2.   Perry VH, Gordon S. Macrophages and microglia in the nervous system.   2013;3:1-6.
              Trends Neurosci 1988;11:273-7.                   28.  López-Cepero JM. Silver Carbonate staining reveals mitochondrial
           3.   PerryVH, Gordon S. Macrophages and the nervous system. Int Rev Cytol   heterogeneity. J Histochem Cytochem 2004;52:211-6.
              1991;125:203-44.                                 29.  Esiri M, Perl D. Oppenheimmer’s diagnostic neuropathology: a practical
           4.   Ling EA, Penney D, Leblond CP. Use of carbon labelling to demonstrate   manual. 3rd ed. London:Hodder-Arnold; 2006.
              the role of blood monocytes as precursors of the ‘amoeboid cells’ in the   30.  Mildner A, Schmidt H, Nitsche M, Merkler D, Hanisch UK, Mack M,
              corpus callosum of postnatal rats. J Comp Neurol 1980;193:631-57.  Heikenwalder M, Bruck W, Priller J, Prinz M. Microglia in the adult
           5.   Streit WJ. Microglia  and  macrophages in  the developing  CNS.   brain arise from Ly-6ChiCCR2+ monocytes only under defined host
              Neurotoxicol 2001;22:619-24.                        conditions. Nat Neurosci 2007;10:1544-53.
           6.   Hanisch UK. Microglia as a source and target of cytokines.  Glia   31.  Carson MJ, Doose JM, Melchior B, Schmid CD, Ploix CC. CNS immune
              2002;40:140-55.                                     privilege: hiding in plain sight. Immunol Rev 2006;213:48-65.
           7.   Graeber MB, Streit WJ. Microglia: Immune Network in the CNS. Brain   32.  Giulian D, Baker TJ. Characterization of amoeboid microglia isolated
              Pathol 1990;1:2-5.                                  from developing mammalian brain. J Neurocsi 1986;6:2163-78.
           8.   Becher B, Prat A, Antel JP. Brain-immune connection: immuno-regulatory   33.  Streit WJ, Walter SA, Pennell NA. Reactive microgliosis. Prog Neurobiol
              properties of CNS-resident cells. Glia 2000;29:293-304.  1999;57:563-81.
           9.   Nimmerjahn A, Kirchhoff F, Helmchen F. Resting microglial cells are   34.  Mittelbronn M. The M1/M2 immune polarization concept in microglia: a
              highly dynamic surveillants of brain parenchyma  in vivo.  Science   fair transfer? Neuroimmunol  Neuroinflammation 2014;1:6-7.
              2005;308:1314-8.                                 35.  Schafer DP, Stevens B. Synapse elimination during development and
           10.  Kettenmann H, Hanisch UK, Noda M, Verkhratsky A. Physiology of   disease: immune molecules take centre stage.  Biochem Soc Trans
              Microglia. Physiol Rev 2011;91:461-553.             2010;38:476-81.
           11.  Luo  XG,  Chen  SD.  The  changing  phenotype  of  microglia  from   36.  Schafer DP, Lehrman EK, Stevens B. The “Quad-Partite” synapse:
              homeostasis to disease. Transl Neurodegener 2012;1:9.  Microglia-synapse interactions in the developing and mature CNS. Glia
           12.  Neumann H, Kotter MR, Franklin RJ. Debris clearance by microglia:an   2013;61:24-36.
              essential link between degeneration and regeneration.  Brain   37.  Harry GJ, Kraft AD. Microglia in the developing brain: A potential target
              2009;132:288-95.                                    with lifetime effects. Neurotoxicology 2012;33:191-206.









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