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Yang et al. J Cancer Metastasis Treat 2017;3:65-70 Journal of
DOI: 10.20517/2394-4722.2016.65
Cancer Metastasis and Treatment

www.jcmtjournal.com
Original Article Open Access

Expression of PANDA, LincRNA-p21, PUMA

in lung tissues of lung cancer patients in

the Xuanwei and non-Xuanwei areas of

Yunnan Province

Kai-Yun Yang , Zhi-Qiang Shen , Yue-Feng He , Kushal Rizal , Hui Tan , An-Ning Chen , Yun-Chao Huang ,
3
3
3
1,3
1
2
3
Guang-Qiang Zhao , Yu-Jie Lei 3
3
1 Pharmacy College of Kunming Medical University, Chenggong, Kunming 650500, Yunnan, China.
2 Public Health College of Kunming Medical University, Chenggong, Kunming 650500, Yunnan, China.
3 The Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan), Kunming 650118, Yunnan, China.
Correspondence to: Prof. Zhi-Qiang Shen, Pharmacy College of Kunming Medical University, Yuhua District Spring Melt Road No. 1168,
Chenggong, Kunming 650500, Yunnan, China. E-mail: 231833488@qq.com
How to cite this article: Yang KY, Shen ZQ, He YF, Rizal K, Tan H, Chen AN, Huang YC, Zhao GQ, Lei YJ. Expression of PANDA, LincRNA-p21,
PUMA in lung tissues of lung cancer patients in the Xuanwei and non-Xuanwei areas of Yunnan Province. J Cancer Metastasis Treat 2017;3:65-70.
ABSTRACT
Article history: Aim: To study the expression of PANDA, LincRNA-p21, and PUMA in lung tissue of
Received: 17-11-2016 patients with lung cancer from Xuanwei of Yunnan Province. Methods: Forty-five
Accepted: 22-02-2017 cases of lung cancer patients from Xuanwei and 42 lung cancer cases from non-Xuanwei
Published: 27-03-2017 were enrolled. Extraction of RNA was done using the Trizol kit. Real-time fluorescence
quantitative PCR assay was done to obtain the relative expression. Results: Expressions
Key words: of PANDA, LincRNA-p21, and PUMA in male and female patients or in squamous cell
Lung cancer, carcinoma and adenocarcinoma were not significantly different (P > 0.05). However,
PANDA, expression of LincRNA-p21 in Xuanwei patients was higher than non-Xuanwei patients
LincRNA-p21, (P < 0.05). Expression of PUMA in tumor tissue was lower than that in normal lung tissue
PUMA, (P < 0.05), and in Xuanwei patients was lower than non-Xuanwei patients (P < 0.05). In
Xuanwei area patients from non-Xuanwei regions, expression of LincRNA-p21 in patients with smoking
index > 400 was higher than in those < 400 and non-smokers. Conclusion: Expressions
of PANDA, LincRNA-p21, and PUMA in lung tissues have no gender differences or tissue
specificity. High expression of LincRNA-p21 in Xuanwei patients may have relationship
with cell damage caused by coal burning pollution in Xuanwei.

INTRODUCTION in the south by Zhanyi and Fuyuan Counties. Xuanwei
has a population of 1,518,500. It has an estimated
Xuanwei is located in the northeast of Yunnan, China. 801,100 males and 717,400 females. The primary
In the east it is bordered by Panxian County of Guizhou, source of income in Xuanwei is from agriculture, coal

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