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Dave et al. Nibrin expression in OSCC

Table 1: Relation of Nibrin immunoreactivity with clinical and pathological parameters in OSCC tissue
Nibrin expression in tumor (Median: 210)
Variables n Weak, n (%) Strong, n (%) Correlation (r) P
Age (year): median 45
< 45 47 22 (47) 25 (53) -0.004 0.972
≥ 45 53 25 (47) 28 (53)
Gender
Male 75 33 (44) 42 (56) +0.104 0.303
Female 25 14 (56) 11 (44)
Anatomic site
Tongue 61 30 (49) 31 (51) +0.055 0.589
Buccal mucosa 39 17 (44) 22 (56)
Tobacco habit
Absent 14 9 (64) 5 (36) +0.140 0.166
Present 86 38 (44) 48 (56)
Disease status (n = 78)
No recurrence 46 23 (50) 23 (50) +0.031 0.789
Recurrence 32 15 (47) 17 (53)
Disease outcome (n = 90)
Alive 51 23 (45) 28 (55) -0.036 0.737
Dead 39 19 (49) 20 (51)
Tumor size
T1 - T2 71 28 (39) 43 (61) -0.237 0.018
T3 - T4 29 19 (66) 10 (34)
Tumor stage
I 20 8 (40) 12 (60) -0.194 0.039
II 22 8 (36) 14 (64)
III 18 6 (33) 12 (67)
IV 40 25 (62) 15 (38)
Nodal status
Negative 59 25 (42) 34 (58) -0.111 0.271
Positive 41 22 (54) 19 (46)
Tumor differentiation
Well 50 29 (58) 21 (42) +0.220 0.028
Moderately/poorly 50 18 (36) 32 (64)
Keratin
Absent 79 34 (43) 45 (57) -0.154 0.126
Present 21 13 (62) 8 (38)
Lymphatic permeation
Absent 91 43 (47) 48 (53) +0.095 0.874
Present 9 4 (44) 5 (56)
Vascular permeation
Absent 99 47 (47) 52 (53) +0.095 0.349
Present 1 0 (0) 1 (100)
Perineural invasion
Absent 82 40 (49) 42 (51) +0.076 0.451
Present 18 7 (39) 11 (61)
Lymphocytic stromal response
Absent 46 21 (46) 25 (54) -0.025 0.806
Present 54 26 (48) 28 (52)
OSCC: oral squamous cell carcinoma

and weak Nibrin expression (45%, 19/42). In relation to DISCUSSION
relapse free survival also we were unable to find any
significant incidence of disease relapse in patients with Nibrin (p95, NBN, NBS1, NBS) is a 754-amino acid
strong (42%, 17/40, log-rank = 0.006, df = 1, P = 0.937) polypeptide which is involved in the recognition and
and weak Nibrin expression (39%, 15/38). Although we the repair of DSBs. [15,27-29] It interacts with Mre11 and
were unable to obtain any significant findings in total RAD50 to form the MRN complex and is required
[29]
patients, we further sub grouped patients into early and for translocation of this complex to sites of DSBs.
advanced stage disease and surprisingly, we observed Although, in advanced head and neck SCC the
that in patients with early stage disease, a significant prognostic significance of over expression of Nibrin
[30]
high incidence of disease relapse was observed in by immunohistochemistry has been identified.
However, data on the correlation of Nibrin with the
patients with strong Nibrin expression (43%, 10/23, clinicopathological prognosticators are limited. So, the
log-rank = 3.884, df = 1, P = 0.049) as compared to current study evaluated correlation between Nibrin
patients with weak Nibrin expression (8%, 1/12) expression with clinicopathological parameters in total
[Table 2 and Figure 2]. No such significant difference 100 patients with SCC of tongue and buccal mucosa.
was noted for overall survival in this subgroup of
patients. On the other hand in patients with advanced In the present study, we observed nuclear expression of
disease, Nibrin expression failed to discriminate such Nibrin in OSCC tissues and its corresponding adjacent
high and low risk sub group patients for survival. normal tissues. However, there was no significant
Journal of Cancer Metastasis and Treatment ¦ Volume 2 ¦ November 25, 2016 439