Page 103 - Read Online

Further studies are needed to investigate the potential of the 12. Berger TG, Haendle I, Schrama D, Luftl M, Bauer N, Pedersen LO,
cytotoxic activity of CD8 cells as a prognostic factor. The Schuler-Thurner B, Hohenberger W, Straten Pt P, Schuler G, Becker JC.
+
cytotoxic activity may also play an important role in tumor Circulation and homing of melanoma-reactive T cells to both cutaneous
and visceral metastases after vaccination with monocyte-derived
recurrence. In addition, the possible correlation between an dendritic cells. Int J Cancer 2004;111:229-37.
active immune response and disease free survival needs to be 13. Erdmann M, Dorrie J, Schaft N, Strasser E, Hendelmeier M, Kampgen E,
clarified. The combination of toll-like receptor agonists with Schuler G, Schuler-Thurner B. Effective clinical-scale production of
RFA has the potential to improve the outcome of patients dendritic cell vaccines by monocyte elutriation directly in medium,
subsequent culture in bags and fi nal antigen loading using peptides or
with solid tumors in the liver. [23] RNA transfection. J Immunother 2007;30:663-74.
14. Schaft N, Dorrie J, Muller I, Beck V, Baumann S, Schunder T,
ACKNOWLEDGMENTS Kampgen E, Schuler G. A new way to generate cytolytic tumor-specifi c
T cells: electroporation of RNA coding for a T cell receptor into T
lymphocytes. Cancer Immunol Immunother 2006;55:1132-41.
This project was supported by the Hans-Loewel foundation Bamberg, 15. Schuler G, Thurner B, Romani N. Dendritic cells: from ignored cells to
Germany. major players in T-cell-mediated immunity. Int Arch Allergy Immunol
1997;112:317-22.
REFERENCES 16. Schuler-Thurner B, Dieckmann D, Keikavoussi P, Bender A, Maczek C,
Jonuleit H, Roder C, Haendle I, Leisgang W, Dunbar R, Cerundolo V,
von Den Driesch P, Knop J, Brocker EB, Enk A, Kampgen E, Schuler G.
1. Solbiati L, Ierace T, Tonolini M, Osti V, Cova L. Radiofrequency thermal Mage-3 and infl uenza-matrix peptide-specifi c cytotoxic T cells are
ablation of hepatic metastases. Eur J Ultrasound 2001;13:149-58. inducible in terminal stage HLA-A2.1+melanoma patients by mature
2. Solbiati L, Livraghi T, Goldberg SN, Ierace T, Meloni F, Dellanoce M, monocyte-derived dendritic cells. J Immunol 2000;165:3492-6.
Cova L, Halpern EF, Gazelle GS. Percutaneous radio-frequency ablation 17. Steinbrink K, Jonuleit H, Muller G, Schuler G, Knop J,
of hepatic metastases from colorectal cancer: long-term results in Enk AH. Interleukin-10-treated human dendritic cells induce a
117 patients. Radiology 2001;221:159-66. melanoma-antigen-specifi c anergy in CD8(+) T cells resulting in a
3. Dewhirst MW, Landon CD, Hofmann CL, Stauffer PR. Novel approaches failure to lyse tumor cells. Blood 1999;93:1634-42.
to treatment of hepatocellular carcinoma and hepatic metastases using 18. Thurner B, Haendle I, Roder C, Dieckmann D, Keikavoussi P,
thermal ablation and thermosensitive liposomes. Surg Oncol Clin N Am Jonuleit H, Bender A, Maczek C, Schreiner D, von den Driesch P,
2013;22:545-61. Brocker EB, Steinman RM, Enk A, Kampgen E, Schuler G. Vaccination
4. Sofocleous CT, Sideras P, Petre EN. “How we do it” - a practical with mage-3A1 peptide-pulsed mature, monocyte-derived dendritic
approach to hepatic metastases ablation techniques. Tech Vasc Interv cells expands specific cytotoxic T cells and induces regression
Radiol 2013;16:219-29. of some metastases in advanced stage IV melanoma. J Exp Med
5. Hansler J, Neureiter D, Strobel D, Muller W, Mutter D, Bernatik T, 1999;190:1669-78.
Hahn EG, Becker D. Cellular and vascular reactions in the liver to 19. Jarver P, Zaghloul EM, Arzumanov AA, Saleh AF, McClorey G,
radio-frequency thermo-ablation with wet needle applicators. Study on Hammond SM, Hallbrink M, Langel U, Smith CI, Wood MJ, Gait MJ,
juvenile domestic pigs. Eur Surg Res 2002;34:357-63. Andaloussi SE. Peptide nanoparticle delivery of charge-neutral
6. Wissniowski TT, Hänsler J, Neureiter D, Frieser M, Schaber S, splice-switching morpholino oligonucleotides. Nucleic Acid Ther
Esslinger B, Voll R, Strobel D, Hahn EG, Schuppan D. Activation of 2015;25:65-77.
tumor-specifi c T lymphocytes by radio-frequency ablation of the VX2 20. Khairuddin N, Gantier MP, Blake SJ, Wu SY, Behlke MA, Williams BR,
hepatoma in rabbits. Cancer Res 2003;63:6496-500. McMillan NA. siRNA-induced immunostimulation through TLR7
7. Behm B, Di Fazio P, Michl P, Neureiter D, Kemmerling R, Hahn EG, promotes antitumoral activity against HPV-driven tumors in vivo.
Strobel D, Gress T, Schuppan D, Wissniowski TT. Additive antitumour Immunol Cell Biol 2012;90:187-96.
response to the rabbit VX2 hepatoma by combined radio frequency 21. van der Bilt JD, Soeters ME, Duyverman AM, Nijkamp MW,
ablation and toll like receptor 9 stimulation. Gut 2014;DOI: 10.1136/ Witteveen PO, van Diest PJ, Kranenburg O, Borel Rinkes IH.
gutjnl-2014-308286. Perinecrotic hypoxia contributes to ischemia/reperfusion-accelerated
8. Hansler J, Wissniowski TT, Schuppan D, Witte A, Bernatik T, Hahn EG, outgrowth of colorectal micrometastases. Am J Pathol
Strobel D. Activation and dramatically increased cytolytic activity of 2007;170:1379-88.
tumor specifi c T lymphocytes after radio-frequency ablation in patients 22. Nijkamp MW, van der Bilt JD, de Bruijn MT, Molenaar IQ,
with hepatocellular carcinoma and colorectal liver metastases. World J Voest EE, van Diest PJ, Kranenburg O, Borel Rinkes IH. Accelerated
Gastroenterol 2006;12:3716-21. perinecrotic outgrowth of colorectal liver metastases following
9. Ocker M, Alajati A, Ganslmayer M, Zopf S, Luders M, Neureiter D, radiofrequency ablation is a hypoxia-driven phenomenon. Ann Surg
Hahn EG, Schuppan D, Herold C. The histone-deacetylase inhibitor 2009;249:814-23.
SAHA potentiates proapoptotic effects of 5-fl uorouracil and irinotecan 23. Prud’homme GJ. DNA vaccination against tumors. J Gene Med
in hepatoma cells. J Cancer Res Clin Oncol 2005;131:385-94. 2005;7:3-17.
10. Di Fazio P, Schneider-Stock R, Neureiter D, Okamoto K, Wissniowski T,
Gahr S, Quint K, Meissnitzer M, Alinger B, Montalbano R, Sass G,
Hohenstein B, Hahn EG, Ocker M. The pan-deacetylase inhibitor How to cite this article: Wissniowski TT, Gress T, Di Fazio P. Long-term
panobinostat inhibits growth of hepatocellular carcinoma models by immune-modulatory side effects of radiofrequency ablation in patients with
alternative pathways of apoptosis. Cell Oncol 2010;32:285-300. liver metastases and hepatocellular carcinoma. Hepatoma Res 2015;1:92-6.
11. Livraghi T, Goldberg SN, Solbiati L, Meloni F, Ierace T, Gazelle GS.
Percutaneous radio-frequency ablation of liver metastases from breast Source of Support: Nil. Confl ict of Interest: None declared.
cancer: initial experience in 24 patients. Radiology 2001;220:145-9.

96 Hepatoma Research | Volume 1 | Issue 2 | July 15, 2015