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Harangi et al. Vessel Plus 2017;1:166-73 Vessel Plus
DOI: 10.20517/2574-1209.2017.27
www.vpjournal.net
Topic: Atherosclerosis and Related Diseases Open Access

HDL subfraction distribution and HDL

function in untreated dyslipidemic

patients

Mariann Harangi, Anita Szentpéteri, Bíborka Nádró, Hajnalka Lőrincz, Ildikó Seres, Dénes Páll, György Paragh

Department of Internal Medicine, University of Debrecen Faculty of Medicine, H-4032 Debrecen, Hungary.
Correspondence to: Dr. Mariann Harangi, Department of Internal Medicine, University of Debrecen Faculty of Medicine, Nagyerdei krt. 98, H-4032
Debrecen, Hungary. E-mail: mharangi@hotmail.com

How to cite this article: Harangi M, Szentpéteri A, Nádró B, Lőrincz H, Seres I, Páll D, Paragh G. HDL subfraction distribution and HDL function
in untreated dyslipidemic patients. Vessel Plus 2017;1:166-73.

ABSTRACT
Article history: Aim: The protective role of high-density lipoprotein (HDL) against atherosclerosis is well
Received: 31 Jul 2017 known. However, both structural and functional changes of the HDL particles may affect its
Accepted: 18 Sep 2017 protective efficacy. Increased levels of HDL-associated myeloperoxidase (MPO) and decreased
Published: 28 Dec 2017 HDL-linked paraoxonase-1 (PON1) activity have been reported in dyslipidemic patients. Some
changes in HDL subfraction distributions were also studied previously, but data on structural
Key words: and functional changes in dyslipidemia are not complete. Therefore, the authors aimed to
High-density lipoprotein, evaluate these qualitative and quantitative markers of HDL in dyslipidemic patients and
subfraction, healthy control subjects. Methods: Anthropometric parameters, serum levels of lipoproteins
paraoxonase,
myeloperoxidase, and MPO, as well as PON1 activities were investigated in 81 untreated dyslipidemic patients
dyslipidemia and in 32 healthy gender-matched controls. Additionally, HDL subfractions were detected
by an electrophoretic method on polyacrylamide gel (Lipoprint). Results: Significantly
higher glucose, hemoglobin A1c, total cholesterol, low-density lipoprotein-cholesterol,
triglyceride, lipoprotein(a), apolipoprotein B, C-reactive protein, and MPO levels were found
in patients compared to the healthy subjects. There were no significant differences in PON1
paraoxonase and arylesterase activities between the two study groups, but MPO/PON1 ratio
was significantly higher in patients. There was a shift towards the smaller HDL subfractions,
but only the intermediate HDL ratio was significantly lower in patients compared to controls.
Conclusion: The results highlight the importance of HDL-associated pro- and antioxidant
enzymes suggesting the possible clinical benefit of MPO/PON1 calculation and confirm that
quantification of HDL-C level alone provides limited data regarding HDL’s cardioprotective
effect. Calculation of MPO/PON1 ratio may be a useful cardiovascular marker in dyslipidemia.

INTRODUCTION heterogeneous in their structural, chemical and
biological properties. Using different analytical
High-density lipoprotein (HDL) is a fraction of methods HDL can be separated to subclasses
small, dense, protein-rich lipoprotein that is highly differing in size, density, shape and lipid and protein

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