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Page 10 of 14 Lust et al. Vessel Plus 2021;5:7 I http://dx.doi.org/10.20517/2574-1209.2020.52
Figure 4. Effect of diclofenac on venous remodeling, in vivo. Imaging of the mice auricular vein vasculature immediately after and
4 days after ligation of the auricle vein revealed an increase in tortuosity and diameter of veins connected to the occluded venous
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network (arrow in A, ***P < 0.001 vs. control 0 d, n = 10). This was significantly inhibited (arrowheads in A, P < 0.01 vs. control 4 d, n
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= 10) by local administration of diclofenac (Voltaren Spray ). Control auricles were treated with an inert solvent. Original diameter of
the remodeling veins was set to 1. Mouse auricle tissue (control, 4 d) was fixed, paraffin-embedded and cross-sections of veins were
analyzed by immunofluorescence staining to detect CD31 (endothelial cell marker) and COX-2 in combination with DAPI. COX-2 was
barely detected in veins undergoing no remodeling process (B and C), but was detectable in endothelial cells of remodeling veins (arrows
in D and E, scale bar: 50 μm).
release of defined prostanoids and the outcome of the corresponding signaling events are controlled by cell-
specific distribution of terminal prostaglandin-synthases and prostaglandin-receptors.
