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Orgun et al. Regenerative mechanisms of adipose-derived stem cells

g

Figure 1: The phenotypic differences between SVF cells and culture expanded ASCs. ASCs: adipose-derived stem cells; SVF: stromal
vascular fraction

adipose tissue. Liposuction is the most widely used function. Results of the study showed no statistically
method to harvest fat from humans. The liposuction significant difference in terms of yield or function of ASCs
aspirate is primarily composed of fragmented fat, harvested from different locations. Jurgens et al.
[19]
blood and saline. ASCs are usually isolated from the compared ASCs harvested from abdomen versus hip/
fatty portion. A previous study showed that isolation of thigh region, and similarly no significant difference
ASCs from the liposuction aspirate fluid (LAF) portion in the yield and function of ASCs was found.
is also possible, but unfortunately this method yields Gnanasegaran et al. [20] has investigated whether
less cells. [14] the harvesting procedure affects isolated ASCs.
They argued that cells harvested using liposuction
The investigation for new methods to increase had endodermal propensity while cells harvested by
efficiency of ASC isolation also needs to be mentioned. excision had meso/ectodermal propensity.
Conventional methods to acquire SVF or ASCs take a
long time, making the process costly and increasing the Preservation of human ASCs for further use is another
risk of contamination. Sachs et al. [15] and Francis et al. [16] subject which is being investigated. According to
isolated viable ASCs from liposuction aspirate within Shah et al. [21] colony forming potential and cell
thirty minutes without using collagenase digestion; marker expression of ASCs except CD34 and CD45
however, the cell yield was much less compared to did not change with cryopreservation; however, their
conventional isolation method. In another study, it is differentiation potential was significantly reduced.
suggested that using fluorescent assisted cell sorting Further investigation on this subject is necessary.
(FACS) for ASCs isolation is possible, however this
method also yields less cells. [17] THE EFFECTS OF ASCS ON ANGIOGENESIS

Schipper et al. investigated whether the region of There is a considerable amount of research on the
[18]
harvest or the age of the donor had effects on ASCs’ angiogenic effects of ASCs against ischemic diseases.
34 Plastic and Aesthetic Research ¦ Volume 4 ¦ March 22, 2017