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Page 33 of 36 J Cancer Metastasis Treat 2019;5:31 I http://dx.doi.org/10.20517/2394-4722.2019.21

On the whole, chemotherapy administration could contribute to muscle wasting in cancer patients,
enhancing the alterations of metabolism due to tumor growth. In this regard, moderate exercise may
support cancer patients under chemotherapy regimen preserving muscle mass and function.

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43. Mouse mammary carcinomas secrete extracellular vesicles - in vitro and in vivo study

Yuko Ito, Nabil Eid, Masa-Aki Shibata, Yoshinori Otsuki, Yoichi Kondo

Department of Anatomy & Cell Biology, Division of Life Sciences, Osaka Medical College, 2-7 Daigaku-
machi, Takatsuki, Osaka, 569-8686, Japan

Many types of cells including cancer cells secrete extracellular vesicles (EVs). EVs are small vesicles
released by donor cells that can be taken up by recipient cells. EVs contain receptor proteins, proteolytic
enzymes, miRNAs, and mRNAs which are transferred into the target cells, and then affect various cell
functions. EVs are categorized depending upon where in the cells they originate: vesicles that are derived
from multivesicular bodies are referred to as exosomes (50-100 nm in diameter) and those from the plasma
membranes as microvesicles (500-1000 nm in diameter). There are numerous in vitro studies, however, little
is known about EVs in vivo. Here we show the chemical and ultrastructural features of EVs derived from
cultured mouse mammary carcinoma cells and their tumors in mice formed upon inoculation. BJMC338
tumors show a low metastatic propensity, while BJMC3879 tumors show a high metastatic propensity,
especially to lymph nodes and lungs. Both EVs contain premature vascular endothelial cell growth factor
C, and inoculated tumors demonstrated that EVs were in their lumen and surrounding connective tissue.
Many exosomes were accumulated in the cytoplasm, Golgi complex and r-ER of tumor cells. These
observations suggest that mouse mammary carcinoma cells in tumors secrete EVs.

44. Epithelial splicing regulatory protein 1 expression is an unfavorable prognostic factor in
prostate cancer patients

2
1
1
2
2
3
Kang Hyun Lee , Sung Han Kim , Andy Jinseok Lee , Weon Seo Park , Jongkeun Park , Jongkeun Lee ,
1
4
Boram Park , Jae Young Joung , Dongwan Hong 2
1 Department of Urology, Center for Prostate Cancer, Goyang-si 410-769, South Korea.
2 Clinical Genomics Analysis Branch, National Cancer Center Korea, Goyang-si 410-769, South Korea.
3 Department of Pathology, Center for Prostate Cancer, 4Biometrics Research Branch and Biostatistics
Collaboration Unit, National Cancer Center, Goyang-si 410-769, South Korea.
The objective of this study was to evaluate the role of epithelial splicing regulatory protein 1 (ESRP1)
expression in predicting prognosis and disease progression in a large cohort of PC patients with long-