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J Cancer Metastasis Treat 2019;5:31 I http://dx.doi.org/10.20517/2394-4722.2019.21 Page 30 of 36
2 Unit of Oncology, Department of Translational Medicine, Università del Piemonte Orientale “A. Avogadro”,
Novara 28100, Italy.

In this work, we investigated whether glucose uptake plays a role in ovarian cancer cell migration. IL-6,
a cytokine released by cancer-associated fibroblasts and cancer cells, promotes ovarian cancer metastasis.
We have previously found that Resveratrol (RV), a naturally polyphenolic compound, counteracts IL-6-
induced cancer cell migration, through the epigenetic up-regulation of autophagy. Here, we show that IL-6
stimulates glucose-uptake along with cell migration. We found that in the presence of glucose, IL-6-induced
mitochondrial oxidative stress with production of anion superoxide and inactivation of ATG4, the cysteine
protease that processes LC3 needed for autophagosome formation. RV reduced glucose uptake, prevented
the formation of anion superoxide, rescued ATG4 function and increased the level of autophagosome
formation. RV-mediated up-regulation of autophagy was more pronounced at the migration front, leading
to a reduced cancer cell motility. These data indicate that IL-6 promotes ovarian cancer cell migration by
promoting glucose uptake which is metabolized to produce mitochondrial oxidative stress that eventually
inhibits autophagy, and RV may counteract this action by limiting the glucose uptake and the production
of anion superoxide.

39. Warty Carcinoma of the uterine cervix - do we know it

5
2
4
1
1
3
Angel Yordanov , Strahil Strashilov , Milena Karcheva , Polina Vasileva , Ilko Iliev , Stanislav Slavchev ,
Yoana Ivanova 4
1 Clinic of Gynecologic Oncology, Medical University Pleven, Pleven 5803, Bulgaria.
2 Department of Plastic Restorative, Reconstructive and Aesthetic Surgery, Medical University Pleven, Pleven
5803, Bulgaria.
3 Department of Epidemiology, Medical University Pleven, Pleven 5803, Bulgaria.
4 Department of Obstetrics and Gynecology, Medical University Pleven, Pleven 5803, Bulgaria.
5 Clinic of Gynaecology, University Hospital “St. Anna”-Varna, Varna 9002, Bulgaria.

Introduction: Warty carcinoma of the uterine cervix is a rare subtype of squamous cell carcinoma that
usually occurs in women over 48-50 years. Warty carcinoma is not as aggressive as the other subtypes, and
has a better prognosis. It is also a slow-growing tumor. The prognosis depends on a set of several factors
that include: the size of the tumor and the extent of its invasion, stage of cancer, FIGO grade, involvement
of the regional lymph nodes, whether the tumor is occurring for the first time, or is a recurrent tumor,
response to treatment.

Aim: The aim of the study was to explore the prognosis of this type of cervical cancer.

Methods: For ten year period (2008-2017) in the Clinic of Gynecologic oncology at the UMHAT - Pleven,
Bulgaria were operated 714 cases with Carcinoma of the uterine cervix and 14 of which were histologically
diagnosed as a Warty carcinoma. Radical abdominal hysterectomy was performed on all patients with
pelvic lymph node dissection with postoperative telegamma therapy. Patients were investigated by retro-
and prospective analysis for overall and recurrence-free survival rate.

Results: Warty carcinoma was 1.94% of all cervical carcinomas, treated in our clinic. The mean age of
the patients was 48 years (29 to 72 years) and 57% of them were peri- and postmenopausal and 43% were
in women under the age of 45. According to the FIGO staging systems for cervical cancer, patients were
staged as follows: in stage IB1 - 43% and in stage IB2 - 57%. In 29% the tumor size was under 2 cm, in 14%