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Liu et al. J Cancer Metastasis Treat 2017;3:169-76 Journal of
DOI: 10.20517/2394-4722.2017.46
Cancer Metastasis and Treatment

www.jcmtjournal.com
Original Article Open Access

The synergy of Helicobacter pylori and lipid

metabolic disorders in induction of Th17-related

cytokines in human gastric cancer

Jie Liu , Han Wang , Gang Chen , Mo Yang , Zhi-Xian Wu , Russell Erick Ericksen , Alice Sze Tsai Wong ,
4
1,3
5
1
1
2
1
Weiping Han , Jin-Zhang Zeng 1
4
1 Fujian Provincial Key Laboratory of Innovative Drug Target Research and State Key Laboratory of Cellular Stress Biology, School of Pharmaceutical
Sciences, Xiamen University, Xiamen 361102, Fujian, China.
2 Department of Pathology, Fujian Provincial Cancer Hospital, Fujian Medical University, Fuzhou 350014, Fujian, China.
3 Department of Hepatobiliary Disease, Fuzhou General Hospital (Dongfang Hospital), Xiamen University, Fuzhou 350025, Fujian, China.
4 Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Singapore 138667, Singapore.
5 School of Biological Sciences, University of Hong Kong, Hong Kong 999077, China.
Correspondence to: Dr. Jin-Zhang Zeng, Fujian Provincial Key Laboratory of Innovative Drug Target Research and State Key Laboratory of
Cellular Stress Biology, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, Fujian, China. E-mail: jzzeng@xmu.edu.cn;
Dr. Weiping Han, Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, Agency for Science, Technology and Research,
Singapore 138667, Singapore. E-mail: Weiping_Han@sbic.a-star.edu.sg
How to cite this article: Liu J, Wang H, Chen G, Yang M, Wu ZX, Ericksen RE, Wong AST, Han W, Zeng JZ. The synergy of Helicobacter pylori
and lipid metabolic disorders in induction of Th17-related cytokines in human gastric cancer. J Cancer Metastasis Treat 2017;3:169-76.
ABSTRACT

Article history: Aim: To study the impact of Helicobacter pylori (H. pylori) and lipid metabolic disorder
Received: 03-07-2017 on the expression of Th17-related cytokines in gastric cancer (GC). Methods: GC
Accepted: 22-08-2017 specimens were randomly collected from 42 patients, of whom 15 had H. pylori infection
Published: 29-08-2017 and 27 were without. Tumor RNA was extracted for reverse transcription quantitative
polymerase chain reaction quantification of gene expression. Results: The mRNA levels
Key words: of interleukin (IL)-6 and leptin, which are known to regulate Th17 differentiation, were
Helicobacter pylori, upregulated by 20 and 6 folds, respectively, in H. pylori-infected compared to uninfected
T helper cells, patients. IL-17A and granulocyte-macrophage colony-stimulating factor, two cytokines
gastric cancer, produced by Th17 cells, were 5- and 6-fold higher in tumors with H. pylori infection,
interleukin-17A, respectively. Consistently, RORγt, a transcription factor regulating Th17 differentiation,
RORγt was increased 6-fold in H. pylori-positive vs. negative tumors. Further elevation of RORγt

was seen in advanced H. pylori-associated tumors. In addition, H. pylori infection was
also associated with enhanced expression of CXCL1 (5 folds), chemotactic factor capable
of driving bone marrow-derived immature myeloid cells. Interestingly, we observed that
H. pylori-associated increase of IL-17A was enhanced in the group with higher plasma
triglycerides. Conclusion: The findings demonstrate a cross-talk and synergistic role of
H. pylori infection and abnormal lipid metabolism in GC development, at least partly via
cooperative induction of Th17 differentiation and activation.

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