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Figure 1: (a) Phase contrast microscope image of CAF; (b) giemsa staining; (c) alizarin staining; (d) oil red staining of CAF. CAF: cancer associated fibroblast

Figure 2: Cell growth curve for control untreated cells vs. Ch-Np-treated cells effect of Ch-Np on alignment of CAF. CAF: cancer associated fibroblast
repeated twice with the same number of cells and using having extended cellular filaments as shown in Figure
same scalpel for making the scratch. 1a. Giemsa Stain stained the nucleus of CAF, as shown
in Figure 1b, making the nucleus completely visible and
RESULTS showing clear cytoplasm. Calcium granules within CAF
cytoplasm of were stained by Alizarin Stain as shown in
Morphological characterization of CAF cells by Figure 1c. Oil Red staining did not impart any color on
phase contrast microscopy the cells as shown in Figure 1d, indicating that there were
Esophageal CAF showed fibroblast-like appearance, no adipocytes.
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Journal of Cancer Metastasis and Treatment ¦ Volume 2 ¦ July 29, 2016 ¦