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Matrix or
Cells Tumor type Condition Experiment scaffold Effect Ref.
Seeding of GBM cells on Increased anti-
LN18, GL15, GBM 2D, 3D brain Hi-spots and exposure Brain Hi-Spots proliferative effect of [65]
U87, A172 to anti-proliferative drugs TMZ on GBM cells
Ara-C, Taxol and TMZ maintained on Hi-spots.
Growth on chitosan-
Comparison of growth
2D, 3D, alginate scaffolds
Matrigel and and morphology and Matrigel, reduces growth but
C6 rat, U-87 Glioma chitosan- secretion of VEGF, MMP2, Chitosan- increases secretion [93]
MG, U-118 MG fibronectin and Laminin alginate
alginate of VEGF, MMP2,
scaffolds between cells grown in 2D, scaffolds fibronectin and
in matrigel or on chitosan- Laminin.
alginate scaffolds.
SAHA treatment causes
2D, 3D Evaluation of SHA impact
LN18, F98, reduction of invasion
F98EGFR- vIII, spheroid on cell growth, collagen and the reorganization
C6 rat, U-87 Glioma culture, I invasion and mRNA Collagen I of the matrix [94]
MG, transwell expression of genes relevant surrounding the tumor
migration for cell-cell and cell-matrix spheroids.
interaction.
Tenascin-C deposition
Analysis of transwell Collagen I triggers glioma invasion
U178, U251 Glioma 3D transwell invasion and migration after supplemented in a PKC∂-dependent [95]
compound inhibition of with Tenascin C manner.
PKC∂.
Analysis of growth Matrix stiffness
and dissemination in Collagen impacts on glioma
U373 Glioma 3D spheroid [19]
increasingly stiff collagen I I-agarose cell invasiveness.
gels. High stiffness blocks
invasiveness.
Interleukin-1 beta (IL-
Quantification of transwell 1b) and tumor necrosis
3D, single migration of cells stimulated factor-alpha (TNF-α)
U251, U178 Glioma Collagen I [96]
cells with TNF-α, IL-1 or a increase glioma cell
combination of both. invasiveness in 3D
with parallel increased
MMP-2 and MMP-9.

Neurosphere compound FDA-approved
Primary mouse Medulloblastoma 3D toxicity assays using FDA- None Pemetrexed and [55]
G3 MB neurospheres approved drugs and ATP- Gemcitabine
sensor dye. significantly block
proliferation of G3 MB.
Quantification of
collagen invasion after
HGF stimulation, small HGF-induced c-Met
2D, 3D
DAOY, UW228 Medulloblastoma transwell, 3D compound kinase inhibitor Collagen I activation promotes MB [9]
micro beads or siRNA treatment in cells cell invasion through
seeded on Micro-beads and the kinase MAP4K4.
embedded in collagen I
matrix.

Quantification of collagen
DAOY, invasion and cell migration HGF, EGF and bFGF
UW228, Med 2D, 3D micro after growth factor are strong promoters of
PDX1712, Medulloblastoma beads and stimulation using invasion Collagen I MB cell migration and [56]
MedPDX411, spheroids counter platform for invasion
primary MB automated quantification
of motile cell behavior in
different environments.
Contind...

Journal of Cancer Metastasis and Treatment ¦ Volume 2 ¦ May 18, 2016 ¦ 155