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Matrix or
Cells Tumor type Condition Experiment scaffold Effect Ref.
Comparison of 2D and ExtracelTM 3D cell culture is
3D cultures for growth better morphological
U87-MG, (Polyethylene-
primary MB GBM 2D, 3D culture and viability after based hydrogel correlate to in vivo [90]
samples irradiation or treatment with HA and tumor, 3D grown
with TMZ, cisplatin or gelatin). GBM moderately less
carmustin. sensitive to irradiation.
3D culture, cells Evaluation of Porous chitosan-HA
U118-MG GBM grown on rigid growth and stem cell Porous chitosan- increases growth and [63]
matrix properties HA causes up-regulation of
stem-cell markers
Embedding
in hydrogels, Hydrogels made HA causes rounded
Patient-
derived tumor GBM 3D, single cells morphology and cell of collagen I, III, morphology and [62]
material migration analysis, or collagen-HA reduces motility of
single cell tracking for mixtures matrix-embedded cells.
motility.
GelMA or
Analysis of growth, PEG4A hydrogels Increasing HAMA
metabolic activity supplemented concentrations cause
U87-MG, Glioma 3D, single cells and HIF-1 VEGF, with up-regulation of [64]
U87+EGFR MMP-2, MMP- methacrylated fibronectin, VEGF and
9 and Fibronectin HA (HAMA) HIF-1.
production. at increasing
concentrations
Analysis of single cell
viability, F-actin size Acrylated HA F-actin reorganization
3D, single cells,
A-172 Glioma microfluidic and cellular orientation cross-linked with and re-orientation of [91]
chip in embedded cells MMP-sensitive or cells in response to
under flow and VEGF RGD peptides flow and VEGF.
in microfluidic chip.
Evaluation of matrix Increased matrix
stiffness impact on Matrigel with stiffness causes
OSU-2 GBM 3D, single cells [32]
tumor cell morphology varying stiffness increased invasive
and migratory/invasive motility.
capabilities.
TMZ showed much
lower cytotoxicity
Evaluation of liver against GBM cells in
M059K, 3D, micro-
HepG2, GBM, scale perfusion cell metabolism on Polylactic acid 3D than in 2D. IFO [68]
CYP3A Hepatoblastoma system cytotoxic effect of IFO scaffold effect dependent on
and TMZ. metabolic activity of
cytochrome P450 in
hepatocytes.
Evaluation of Rho-
family GTPase Perivascular and
activation during intraparenchymal
U251MG, 3D, organotypic GBM invasion in Matrigel, slice invasion is associated
LN229 and GBM slice culture brain slice and 3D culture with increased Rac and [53]
U87MG matrigel culture. Use Cdc42 and reduce Rho
of Rho-family GTPase GTPase activity.
fluorescent protein
sensors.
Differential expression
Comparison of protein of invasion, survival
U87, U251HF,
SNB19, expression in cells and hypoxia driver
LNZ308, Glioma 2D, 3D grown under 2D or AlgiMatrix proteins between 2D [92]
LN229 3D conditions and in and 3D. Effect of 3D
different oxygenation. growth dominates
oxygenation.
Contind...

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Journal of Cancer Metastasis and Treatment ¦ Volume 2 ¦ May 18, 2016 ¦