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B
Figure 1. Selection of MCF-7 and MDA-MB-231 stable transfectants and coimmunoprecipitation of fibulin-1 with ADAMTS-1. A: Western
blot analysis of MCF-7 and MDA-MB-231 expressing exogenous fibulin-1, ADAMTS-1 or both proteins simultaneously (Fibulin-1/
ADAMTS-1). Control, cells transfected with an empty vector. Actin was used as a loading control. Molecular weight marker is indicated on
the right. Independent gels were performed for each western blot; B: immunoprecipitation of MCF-7 control cell extracts or extracts from
cells expressing fibulin-1 and ADAMTS-1 (Fibulin-1/ADAMTS-1) using an anti-FLAG M2 affinity gel. IP, immunoprecipitated. Top, detection
with an anti-fibulin-1 antibody. Bottom, detection with an anti-FLAG (ADAMTS-1) antibody. Intervening irrelevant lanes are not shown
