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Page 8 of 15 Mohamedi et al. J Cancer Metastasis Treat 2019;5:37 I http://dx.doi.org/10.20517/2394-4722.2018.81

Figure 3. Coexpression of ADAMTS-1 and fibulin-1 reduces the migration properties of the breast cancer cell line MCF-7 over type-I
collagen and fibronectin. Top, Representative images of the migration of MCF-7 transfectants on wells coated with type-I collagen and
fibronectin using the RadiusTM 24-Well Cell Migration Assay kit (Cell Biolabs). MCF-7 cells transfected with an empty vector were used
as a control. Pictures of the initial (t: 0 h) and final (t: 24 h) times are included. The starting point is indicated with a thin dotted line, and
the final point is indicated with a thick dotted line. Bottom, graphical representation of percentages of the area covered after 24 h (from
three independent experiments)

of the double transfectants (fibulin-1 and ADAMTS-1) were significantly compromised compared with those
of their respective controls. We observed that the control MCF-7 cells were able to cover 79% of the area on
type-1 collagen-coated dishes, whereas the double transfectants only were able to cover 25% of the area. In