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Della Porta et al. Comparison of p53 and prohibitin expression

Table 2: Comparative TNFR1, TRAIL, NFkB, nuclear RelA/p65, p53, nuclear PHB, cytoplasmic PHB and TUNEL
expression by NL, CIR, HCV, and HCC
NL HCV CIR HCC
Markers Expression P < 0.05
(n = 20) (n = 30) (n = 30) (n = 60)
TNFR1 Negative 18 3 3 32
+ 2 24 9 13 CIR vs. HCC
CIR vs. HCV
++ 0 3 15 12 HCV vs. NL
+++ 0 0 3 3
TRAIL Negative 18 12 9 14
+ 2 18 21 18 HCC vs. CIR
HCC vs. HCV
++ 0 0 0 18 HCV vs. NL
+++ 0 0 0 10
RelA/p65 Negative 16 3 0 21
+ 4 18 9 19 CIR vs. HCC
CIR vs. HCV
++ 0 9 15 13 HCV vs. NL
+++ 0 0 6 7
NFkB Negative 18 0 3 26
+ 2 6 18 22 CIR vs. HCC
CIR vs. HCV
++ 0 15 6 10 HCV vs. NL
+++ 0 9 3 2
p53 Negative 17 27 27 20
+ 3 3 3 26 HCC vs. CIR
HCC vs. HCV
++ 0 0 0 12 HCC vs. NL
+++ 0 0 0 2
Cytoplasmic PHB Negative 16 3 3 35
+ 4 5 7 23 CIR vs. HCC
CIR vs. HCV
++ 0 10 11 2 HCV vs. NL
+++ 0 12 9 0
Nuclear PHB Negative 18 27 24 19
+ 2 3 6 24 HCC vs. CIR
HCC vs. HCV
++ 0 0 0 13 HCC vs. NL
+++ 0 0 0 4
TUNEL Negative 17 3 2 4
+ 3 16 18 15 HCC vs. CIR
HCC vs. HCV
++ 0 11 10 32 HCC vs. NL
+++ 0 0 0 9
TNFR1: tumor necrosis factor receptor-1; TRAIL: TNF-related apoptosis-inducing ligand; NFkB: nuclear factor kappa B; PHB: prohibitin;
TUNEL: transferase-mediated dUTP-biotin nick-end labelling; NL: normal liver; CIR: cirrhosis; HCV: hepatitis C virus; HCC: hepatocellular
carcinoma
The percentage of apoptosis was increased in HCC hepatic cancer development, which is dependent
[Figure 2L] in contrast to CIR [Figure 2K] and HCV on different pathogenic mechanisms of the tumour
[Figure 2J] (P = 0.0054 and P = 0.0217, respectively). microenvironment.
Moreover, the percentage of apoptosis was increased
in HCV in relation to NL [Figure 2I] (P = 0.0161) [Figure 2O]. TNFα cytokine is increased in patients with chronic
HCV infection. [19] TNFα and TNF-receptors levels
DISCUSSION are augmented during chronic HCV infection and
result in disease progression. [20] Similarly, we found a
The findings of the present study reveal the progressive increase of TNFR1 expression in cases
importance of p53 and PHB participation in human of HCV and CIR in comparison to NL. Indeed, TNFα

Hepatoma Research ¦ Volume 3 ¦ February 28, 2017 37