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Feng et al. Hepatoma Res 2021;7:3 I http://dx.doi.org/10.20517/2394-5079.2020.107 Page 7 of 15

Table 2. Representative Oncolytic therapy used in preclinical studies
Virus strain Modification Therapeutic gene HCC cell lines Animal model Dose Ref.
7
Recombinant VSV- Replaced of hemagglutinin- None HepG2 Huh7 NOD.CB17- 10 TCID50,IV [54]
NDV, L289A neuraminidase (HN) prkdcscid/NCrCrl
(NOD-SCID).
5
Getah-like Insertion of valosin- XBP1 Hep3B Hep3B xenografts, 5 × 10 PFUs, IV [55]
9
alphavirus, M1 containing protein (VCP) Nonhuman primate 1 × 10 PFUs, IV
inhibitors Macaca fascicularis.
7
HSV, d0-GFP Mutated in glycoprotein K None Huh7, SMMC7721, Huh7 and Hep3B 1 × 10 PFU, IV
and glycoprotein B QGY7703, xenografts BALB/c.
L-02, BEL7404,
GSG7701, HCCLM3,
MHHC97H, H22
Ad5 Insertion of Golgi protein SphK1 Huh7, HL-7702 Huh7 xenografts 6 × 10 PFU, IT
8
73 (GP73) promoter BALB/c nude mice.
and sphingosine kinase 1
(SphK1)-short hairpin RNA
(shRNA)
9
Recombinant deletion in NS and insertion hGM-CSF MDCK, A549, HepG2 xenografts 2 × 10 PFU, IT [56]
influenza viral, PR8 of h GM-CSF SMCC7721,HepG2 BALB/c nude mice.
6
MeV, MV-Edm None None CC-LM3, MHCC- LM3 xenografts 5 × 10 PFU, IT [57]
97H BALB/c nude mice.
8
Ad, Ad-sp Insertion of Vestigial-Like VGLL4 Hep3B, Huh-7 Huh-7 xenografts 5 × 10 PFU, IT [58]
Family Member 4 (VGLL4) BALB/c nude mice.
6
HSV, HSV T-01 α47 and γ34.5 loci are None HuH-7, Li-7 JHH-1, Hepa1-6 xenografts 2 × 10 PFU, IT [59]
deleted and the LacZ gene JHH2, JHH5, JHH6, BALB/c nude mice.
replaces the ICP6 gene JHH7, HLE, HLF,
PLC/PRF/5, huH-1
10
Ad, Ad-ΔB Insertion of ING4 and ING4 and TRAIL Hep3B Hep3B xenografts 1 × 10 PFU, IV [60]
TRAIL BALB/c nude mice.
Ad, Ad-wnt- Insertion of TSLC1 Wnt and Rb MHCC-97H, PLC/ PLC/PRF/5 6 × 10 PFU, IT [61]
8
E1A(Δ24bp)-TSLC1 pathway PRF/5 xenografts BALB/c
nude mice.
Ad, OAV SG655- Insertion of 11R-P53 and 11R-P53 and GM- Hep3B-C, ECCG5 ECCG5 xenografts Unknow [62]
mGMP GM-CSF CSF BALB/c mice
8
Ad, Ad-ΔB/TRAIL Mutated in E1A and deleted hTRAIL or hIL-12 Hep3B and HuH7 Athymic nude mice, 2 × 10 PFU, IV 1 [63]
10
and Ad-ΔB/IL-12 in E1B regions. Insertion of orthotopic model × 10 PFU, IV
hTRAIL or hIL-12
MeV, (Res + MeV) Encoding of GFP as a None HepG2 and Hep3B No animal model Various MOIs [64]
marker gene and SCD as used
suicide gene
5
VV, GLV-2b-372 Deletion of TK and insertion None Huh-7, Hep G2, SNU- Athymic nude mice 1 × 10 PFU, IT [65]
of TurboFP635 gene 449, and SNU-739 Huh-7 xenograft
VV, GLV-1 h68 Deletion of TK and insertion None Huh-7, Hep 3B, SNU- No animal model Various MOIs [66]
of Renilla luciferasegreen 449 and SNU-739 used
8
Ad, Telomelysin hTERT inserted upstream of hTERT Human: Huh- HBx transgenic mice, 1.25 × 10 PFU, [67]
the E1 gene 7, Hep3B, PLC5, orthotopic model IT
HA22T, HCC36, 6.25 × 10 PFU,
8
and HepG2 Mouse: IT
Hepa-1c1c7 and 3.0 × 10 PFU,
8
Hepa 1-6 IT
7
HSV, G47Δ ICP47 and γ34.5-deletion None HepG2, HepB, Balb/c nude mice 2 × 10 PFU, IT [68]
SMMC-7721, BEL- SMMC-7721, BEL-
7404, and BEL-7405 7404 xenograft
6
HSV, LCSOV Viral glycoprotein H gene miR122, miR-124a HuH-7, HepG2, and Hsd: athymic (nu/ 5 × 10 PFU, IT [69]
linked with liver-specific and let-7 Hep3B nu) mice, Hep3B
apolipoprotein E (apoE)- xenograft
AAT promoter. miR-122a
complimentary sequence to
the 3′ untranslated region
(3′UTR). miR-124a and let-
7 also inserted at 3′ UTR
6
VV, GLV-1 h68 Deletion of TK and insertion None HuH7 and PLC/ Athymic Nude- 5 × 10 PFU, IV [70]
of Renilla luciferasegreen PRF/5 Foxn1nu HuH7 and
fluorescent protein (Ruc- PLC xenografts
GFP), β-galactosidase,
β-glucuronidase