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Han et al. Cancer Drug Resist 2024;7:16 https://dx.doi.org/10.20517/cdr.2024.01 Page 19 of 25

Figure 9. (A) Prognosis of low and high expression levels of hsa-mir-1286a in grade 4 gliomas; (B) Expression of hsa-mir-1286a was
*
**
detected using RT-qPCR ( P < 0.05, P < 0.01 vs. control group); (C) Expression of hsa-mir-1286a after using hsa-miR-1286a inhibitor
**
was detected using RT-qPCR ( P < 0.01 vs. control group); (D) Cell viability of U251 cells after 24 h of TMZ treatment after microFF
hsa-miR-1286 inhibitor added ( **** P < 0.0001 vs. control group). RT-qPCR: Reverse transcription-quantitative polymerase chain
reaction; TMZ: temozolomide.
were identified as the pivotal gene targets within these pathways, respectively. Previous studies have shown
that the development, progression, and deterioration of various tumors are associated with the activation of
the PI3K/Akt signaling pathway [2,29] . The MAPK signaling pathway, comprising several crucial signaling
components and phosphorylation events, plays a pivotal role in tumorigenesis, including breast cancer
[30]
and glioma . Therefore, isocuB may exert its anti-glioma effect through the PI3K/AKT and MAPK
[31]
signaling pathways. Molecular docking is a technique used in drug design to simulate receptor-drug
interaction patterns. In recent years, the utilization of molecular docking to elucidate relevant mechanisms
of action has become a trend in new drug development. Our results showed that isocuB exhibited a strong