Page 257 - Read Online

Page 6 of 9 Nemtsova et al. Vessel Plus 2018;2:27 I http://dx.doi.org/10.20517/2574-1209.2018.48

Table 1. Age-associated comparative assessment of oxidative-antioxidant systems in patients with H and DM2T
Index 2a subgroup (n = 59) 2b subgroup (n = 97)
8-OH-dG, ng/L 14.07 ± 1.03 17.23 ± 0.97*
GPO, μmol/min/gHb 5.73 ± 0.36 5.23 ± 0.23
MDA, μmol/L 7.07 ± 0.44 6.25 ± 0.33
SH-groups, μmol/L 584.38 ± 14.56 567.36 ± 15.03
CIMT RCCA, mm 0.08 ± 0.00 0.08 ± 0.00
CIMT LCCA, mm 0.08 ± 0.00 0.08 ± 0.00
Insulin, μIU/mL 24.55 ± 4.24 19.71 ± 2.17
HOMA-IR 8.88 ± 1.15 7.92 ± 0.86
SBP, mmHg 152.92 ± 5.85 143.64 ± 2.82
DBP, mmHg 92.50 ± 3.29 88.88 ± 1.75
PBP, mmHg 60.42 ± 3.72 54.76 ± 2.15

*P < 0.05: compared to the 2a group; HOMA-IR: insulin resistance index; MDA: malonic dialdehyde; GPO: glutathione peroxidase; SH-
groups: sulfhydryl groups; 8-OH-dG: 8-hydroxy-2-deoxyguanosine; CIMT RCCA: carotid intima-media thickness of right common carotid
artery; CIMT LCCA: carotid intima-media thickness of left common carotid artery; SBP: systolic blood pressure; DBP: diastolic blood
pressure; PBP: pulse blood pressure

with control group, and in 67% not reach the threshold for the European recommendations (CIMT thickness ≤
[14]
0.9 mm) . The subdivision of patients with HT and T2DM into age groups was accompanied by a slight
increase in this parameter, which did not reach significance (2a subgroup - CIMT of right common carotid
artery (RCCA) - 0.086 ± 0.004 mm, CIMT of left common carotid artery (LCCA) - 0.086 ± 0.003 mm, 2b
subgroup - CIMT RCCA - 0.088 ± 0.003 mm and CIMT LCCA - 0.087 ± 0.002 mm, respectively, P > 0.05).
Ultrasound examination of carotid arteries revealed the presence of atherosclerotic plaques in 39 patients
(30.95%), 5 of which (12.82%) belong to the 2a subgroup, and 34 patients (87.18%) - 2b subgroup.

Oxidative stress is defined as an imbalance between the concentration of oxidation products and the
activity of antioxidant processes in the body. Oxidative stress promotes the oxidation of a number of
molecules, such as DNA, lipids, and proteins, which are associated with various processes, including aging.

[16]
[15]
Despite the available data on the beneficial effect of modern antihypertensive and antidiabetic therapy
on oxidative stress, the results of our study demonstrate the presence of intensive OS in patients with the
combined course of HT and T2DM. This manifests in a significant increase in MDA levels (P < 0.05), and a
decrease in levels of SH-groups (P < 0.05) compared with healthy volunteers.

MDA is the main end product in the process of lipid peroxidation. Data from recent years suggest using
the MDA level as a marker of the risk of complications in patients with T2DM, especially inadequately
[17]
compensated .

[18]
In the studies of Carracedo et al. it was shown that people with age showed a significant increase in MDA.
In our work, patients in the older age group had lower MDA values than those under 60 (6.25 ± 0.33 μmoL/L
and 7.07 ± 0.44 μmoL/L, respectively, P > 0.05).

The main role in protecting against the influence of OS is played by an antioxidant system (AOS), one of the
main components of which is the thiol disulfide system. An important biomarker reflecting the state of this
system is the organism thiol status. Thiol status indicates the total level of SH-groups of proteins and free SH-
groups. In patients with the combined course of HT and T2DM [Table 2], there was a significant decrease
in thiol status in comparison with the control group (573.52 ± 10.91 μmoL/L and 712.26 ± 11.08 mmoL/L,
respectively, P < 0.05).