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Purkait et al. Vessel Plus 2018;2:19 Vessel Plus
DOI: 10.20517/2574-1209.2018.16

Original Article Open Access

Genetic variants of renin on the prevalence of
diabetic nephropathy

Pulakes Purkait 1,3,4 , Kalpataru Halder , Jammigumpula Masthanaiah Naidu , Biswanath Sarkar 4
3
2
1 Department of Anthropology, Panjab University, Chandigarh 160014, India.
2 Department of Molecular Biology, Brahmananda Keshab Chandra College, BonHooghly, Kolkata 700108, India.
3 Department of Anthropology, Andhra University, Visakhapatnam 530003, India.
4 DNALaboratory, Anthropological Survey of India, Kolkata 700016, India.
Correspondence to: Dr. Pulakes Purkait, Department of Anthropology, Panjab University, Chandigarh 160014, India.
E-mail: pp.diabetes@gmail.com

How to cite this article: Purkait P, Halder K, Naidu JM, Sarkar B. Genetic variants of renin on the prevalence of diabetic nephropathy.
Vessel Plus 2018;2:19. http://dx.doi.org/10.20517/2574-1209.2018.16

Received: 2 Mar 2018 First Decision: 29 Jun 2018 Revised: 19 Jul 2018 Accepted: 25 Jul 2018 Published: 14 Aug 2018
Science Editor: Alexander D. Verin Copy Editor: Jun-Yao Li Production Editor: Cai-Hong Wang

Abstract
Aim: Renin, a component of the Renin-Angiotensin-Aldosterone System (RAAS), is produced in the juxtaglomerular
cells of the kidney. It is an important factor for the regulation of blood pressure and electrolyte balance and encoded by
the REN gene. Recent studies suggest that the RAAS is a regulator of kidney functions. Individuals with REN variants
have been associated with high blood pressure. We substantiated the hypothesis that genetic variants of REN gene
have significant association with prevalence of nephropathy and in the development of nephropathy in type 2 diabetes
mellitus (T2DM).

Methods: We enrolled to the study 718 consecutive subjects who were registered patients in two individual hospitals in
Kolkata city, India. They consisted of 246 (34.26%) T2DM patients without nephropathy cases, 168 (23.40%) type 2
diabetes with nephropathy cases (T2DNH) and 304 (42.34%) healthy controls. Genotypes were assayed with genomic
DNA for two known variants of the REN gene, i.e., rs16853055 and rs41317140 using sequencing methods.

Results: Association between the REN gene variants and prevalence of T2DM and T2DNH was tested. A significant
association of T2DNH and variant rs41317140 was obtained and it was evident that the rs41317140 (C>T) shows a
2
significant difference between T2DM and T2DNH (x = 4.92; P = 0.03; OR = 0.6162; 95% CI: 0.4006-0.948). The
results from the multiple model test that additive model predicted the association at genotype level and shows a
significant difference between T2DM and T2DNH (OR = 0.6067; P = 0.03). There was no significant association
between T2DNH or T2DM and variant rs16853055.

© The Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0
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