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Wu et al. Mast cells and vein graft remodelling

but suppressed elastin deposition by 6.5 fold. Local neointima formation in normolipidaemic mice 4
mast cell reconstitution (4 weeks before vein graft weeks after grafting (1.7 × 10 ± 0.2 × 10 vs. 1.1
5
5
implantation) rescued the impaired elastin deposition × 10 ± 0.1 × 10 µm ; C57BL/6J vs. Kit W-sh/W-sh ; P
5
5
2
in Kit W-sh/W-sh vein grafts (23.5 ± 4.8 vs. 3.6 ± 1.1 vs. < 0.01 by Student’s t-test), but induced a 2-fold
26.4 ± 4.4 arbitrary units/mm ; C57BL/6J vs. increase in the hyperlipidaemic mice (1.9 × 10
2
5
Kit W-sh/W-sh vs. Kit W-sh/W-sh + lcMC; P < 0.01 by one-way ± 0.2 × 10 vs. 4.2 × 10 ± 0.8 × 10 µm ; apoE
5
2
-/-
5
5
ANOVA). Mast cell deficiency in hyperlipidaemic mice -/- W-sh/W-sh
induced a similar suppression of neointimal elastin vs. apoE Kit ; P < 0.01 by Student’s t-test;
(37.6 ± 4.0 vs. 13.4 ± 2.9 arbitrary units/mm ; apoE Figure 2A and B). Cell proliferation within the vein
-/-
2
vs. apoE Kit W-sh/W-sh ; P < 0.01 by Student’s t-test). graft at 4 weeks was very low in both C57BL/6J
-/-
and Kit W-sh/W-sh (2.1 ± 0.5% vs. 1.8 ± 0.3%; C57BL/6J
Mast cell deficiency increased chronic vs. Kit W-sh/W-sh ), but significantly elevated in apoE -/-
neointimal proliferation and thickening in Kit W-sh/W-sh compared to apoE (1.9 ± 0.5% vs. 5.3 ±
-/-
apoE mice but not in C57BL/6J mice 1.4%; apoE vs. apoE Kit W-sh/W-sh ; P < 0.05 by Mann
-/-
-/-
-/-
Mast cell deficiency moderately suppressed Whitney test; Figure 2A and C).

Figure 1: Mast cell deficiency suppressed elastin deposition in vein graft. (A) Picrosirus red staining demonstrated that both C57BL/6J and
Kit W-sh/W-sh vein grafts synthesised large amount of collagen (in green, yellow and red colour) in the adventitia and perivascular connective
tissue by 4 weeks. No statistical difference was detected in collagen content between groups; (B and C) Elastin deposition was restricted
to the vein graft neointima (visualised by Verhoeff Van Gieson staining, indicated by arrows). Mast cell deficient Kit W-sh/W-sh vein grafts
contained a significantly lower level of elastin versus congenic C57BL/6J vein grafts, which was rescued by local mast cell reconstitution.
Mast cell deficiency in hyperlipidaemic mice (apoE ) also significantly suppressed elastin deposition in the vein graft by 4 weeks. n = 6; **P
-/-
< 0.01. One-way analysis of variance plus Tukey post hoc test was used for multiple group comparison and unpaired student’s t-test for two
group comparison
140 Vessel Plus ¦ Volume 1 ¦ September 26, 2017