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Ruiz-Moya et al.                                                                                                                               Assessment of DIEP flap using CTA with 3D reconstruction

           reconstruction, that  did not  exhibit previous nor   a Digital Imaging and Communications in Medicine
           other  vascular complication, such  as arteriovenous   (DICOM) compatible file on a CD-ROM to be uploaded
                                                                                                    ®
           thrombosis or necrosis.                            to  a  personal  computer  with  the  AYRA   software
                                                              (formerly known as VirSSPA ; Andalusian  Health
                                                                                          ®
           Thirty-seven reconstructions  were excluded  as the   Department, Seville, Spain). The 3D reconstructions
           preoperative perforator mapping was performed with a   of  the  abdominal  wall  were generated  using  the
           hand-held Doppler probe. Four flaps that had exhibited   DICOM  files  by  means  of  the  virtual  reality  AYRA
                                                                                                             ®
           vascular complications  different to diffuse venous   software. All the variables were assessed in these 3D
           congestion  (2 cases of intraoperative  partial  venous   virtual models.
           congestion related to abdominal midline scars, 1 case
           of intraoperative venous thrombosis, and 1 case of late   Evaluation of the images
           venous thrombosis 6 days following the surgery) were   The preoperative 3D reconstruction of each case
           discarded  as  well.  The  final  sample  group  included   was retrieved by the same observer (A. Ruiz-
           data from 169 DIEP flaps. According to the inclusion   Moya).  The following anatomical variables were
           criteria, 7 cases were identified as diffuse congestive   retrospectively analyzed in both groups: the existence
           flaps [Figure 1]. Due to the limited number of cases,   of direct communications between the SIEV and the
           3 controls per case (21 controls) were selected by   perforators  of  the  flap  [Figure 2], the existence of
           computer randomization, in an attempt to control the   communications of both SIEVs across the abdominal
           power of the study and to avoid selection bias.    midline [Figure 3], the 8-cm-diameter SIEV caudal to
                                                              the most superior aspect of the iliac crests [Figure 4],
           Imaging procedures                                 the number of branches of the SIEV, the number of
           The studies of CTA were carried out by a 16-detector-  perforators included in each flap [Figure 5], and the
           row computed tomography scanner (General Electric   flap subcutaneous tissue thickness at a point located
           Light-Speed 16; General Electric Company, Fairfield,
           Conn.).  The parameters followed by the CT scans
           were: 0.37 s rotational speed of the gantry, 0.63 mm
           collimator width  slice thickness, and 1.37 helical
           detector pitch. The voltage of the X-ray tube was 120 kV
           and tube current was 250 to 300 mA. Prior to scanning,
           all patients received an intravenous administration
           of  100 mL  of  nonionic  iodinated  contrast medium at
           a concentration of 350 mg/mL (Omnipaque 350; GE
           Healthcare, Barcelona, Spain) into an antecubital vein.
           Sections of 0.63 in width were obtained at an 0.5-mm
           interval from 4 cm above the umbilicus to the minor
           trochanter of the hip. The resulting set of images was
           automatically  transferred  to  a  computer  workstation,
           which generated multiplanar reformatted images and   Figure 1: Deep inferior epigastric artery perforator flap exhibiting
           3D  volume-rendered  images.  Data  were  stored  as   diffuse venous congestion



















           Figure 2: Three-dimensional abdominal wall reconstruction with AYRA software from computed tomography angiography images. (A) Point
           of assessment (circle) of direct communications between perforators (red) and superficial venous system (blue); (B) direct communications
           viewed from the abdominal wall
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