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β-catenin APC GSK-3β
M Con 3 5 7 9 M Con 3 5 7 9 M Con 3 5 7 9
500 bp
100 bp
Axin LEF1 GAPDH
M Con 3 5 7 9 M Con 3 5 7 9 M Con 3 5 7 9
100 bp
Figure 11: Effect of 10 mmol/L LiCl on β-catenin, APC, GSK-3β, axin, and LEF1 mRNA expression. APC: adenomatous polyposis coli; GSK-3: glycogen
synthase kinase-3β; LEF1: lymphoid enhancer factor-1.
β-catenin APC GSK-3β
M Con 3 5 7 9 M Con 3 5 7 9 M Con 3 5 7 9
100 bp
Axin LEF1 GAPDH
M Con 3 5 7 9 M Con 3 5 7 9 M Con 3 5 7 9
100 bp
Figure 12: Effect of 10 µg/L KGF on β-catenin, APC, GSK-3β, axin, and LEF1 expression. KGF: keratinocyte growth factor; APC: adenomatous polyposis coli;
GSK-3: glycogen synthase kinase-3β; LEF1: lymphoid enhancer factor-1.
culture, DNA quantitation in 25 mmol/L group compared Effect of KGF on HFSC proliferation
with 0 mmol/L group, P < 0.05, as compared with other The proliferation rate of HFSCs increased following KGF
groups, P > 0.05; after 72 h of culture, DNA quantitation treatment, with a direct correlation between KGF dose and
in 25 mmol/L group compared with 0 mmol/L group, P < HFSC proliferation rate being observed [Figures 8 and 9]. HFSCs
0.01, as compared with other groups P > 0.05; after 96 h proliferated well at KGF concentrations less than 50 µg/L. By contrast,
of culture, DNA quantitation in 25 mmol/L group compared at very high KGF concentrations HFSCs proliferated well at
with 0 mmol/L group, P < 0.01, as compared with other early time points, but died prematurely at later time points.
At KGF concentrations of 10-25 µg/L, cells proliferated well
groups, P < 0.05, also representing a significant difference; and showed no signs of premature aging at later time points.
after 120 h of culture, DNA quantitation in 25 mmol/L group
compared with 0 mmol/L group P < 0.05, as compared with Statistical analyses showed that after 24 h of culture, there
other groups P > 0.05. were no significant differences between the KGF-dose
44 Plast Aesthet Res || Vol 3 || Issue 2 || Feb 29, 2016
