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A B
Figure 3: Histopathology of granulation tissue on days 0 and 10 in
the conventional group. A: (a) Conventional group ‑ day 0 ‑ (arrow)
abundant inflammatory cells, (b) minimal blood capillaries, (c) poor
matrix (photographed with an Olympus PM20 photomicroscope ×20);
B: (a) Conventional group ‑ day 10 ‑ (arrow) More number of fibroblast
cells, (b) poorly developed matrix with a minimal number of fibroblasts, Figure 4: Histopathologic scores of necrotic tissue, inflammatory cells,
(c) minimal blood capillaries. (photographed with an Olympus PM20 new blood vessels, and extracellular matrix deposit in limited access
photomicroscope ×20) dressing versus conventional groups (mean ± SE, P < 0.05)

Table 2: Histopathologic scores of LAD and conventional groups
Parameters (n = 42) (mean ± SE) P
LAD group Conventional dressing group
Day 0 Day 10 Day (0‑10) Day 0 Day 10 Day (0‑10)
Necrotic tissue 25.5 ± 0.86 14.0 ± 0.39 11.5 ± 0.48 26.1 ± 0.76 16.0 ± 0.46 10.1 ± 0.30 0.007*
Inflammatory cells 25.0 ± 0.74 12.4 ± 0.45 12.6 ± 0.60 17.0 ± 0.34 8.37 ± 0.35 8.63 ± 0.35 0.018*
New blood vessels 8.3 ± 0.45 21.1 ± 0.63 12.8 ± 0.58 8.0 ± 0.24 17.3 ± 0.56 9.3 ± 0.29 0.005*
ECM deposit 9.7 ± 0.67 23.0 ± 0.78 13.3 ± 0.50 8.4 ± 0.29 18.0 ± 0.46 9.6 ± 0.24 0.001*
Each parameter was assessed individually in 8 microscopic fields per slide. The scores of the 8 microscopic fields were totaled, and the mean ± SE were
calculated, respectively; *P < 0.05 significant difference. Histological scales: 0, no evidence; 1, occasional evidence; 2, light scattering; 3, abundant evidence, and
4, confluent cells or fibers. LAD: Limited access dressing, SE: Standard error, ECM: Extracellular matrix

which form the principal component of connective tissue Financial support and sponsorship
and play a central role in wound healing by providing a Nil.
structural framework for regenerating tissue. [12]
Conflicts of interest
Borgquist et al. showed that chronic wounds treated There are no conflicts of interest.
[13]
with NPWT promote the formation of granulation tissue
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