Page 40 - Read Online

Page 4 of 14 Nakamoto et al. Plast Aesthet Res 2024;11:54 https://dx.doi.org/10.20517/2347-9264.2024.82

Figure 1. Study design of grafted full-thickness burn wound in ovine model that closely mimics clinical scenario. The full-thickness burn
was induced by fire flame (below left). Split-thickness skin grafts were harvested to be meshed and grafted (below center). Wounds in
2
the treatment group received topical application of timolol (0.02 ml/cm of 0.5%), and wounds in the control group received saline in a
similar manner. The groups were assigned to allow comparison of contralateral sites as pairs in order to avoid anatomic location-
dependent wound healing variations (below right).

Figure 2. Digital photographs of the wounds of each sheep in the treatment and control groups at the time of mesh skin graft (Day 0).

staples. These dressings were removed on Day 7 [Figure 3]. Wounds were washed with saline and cleaned
gently with a sharp spoon. After the application of either timolol or the saline vehicle, the wounds were
covered with petroleum jelly and non-adherent pads (Telfa, COVIDIEN, MA). These dressing changes were
performed once a day thereafter up to Day 14. On Day 14, animals were euthanized under deep anesthesia
with intravenous injections of ketamine (40 mg/kg), xylazine (3.0 mg/kg), and analgesia [buprenorphine