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Darbinyan et al. Neuroimmunol Neuroinflammation 2018;5:41 Neuroimmunology and
DOI: 10.20517/2347-8659.2018.33 Neuroinflammation

Original Article Open Access

Changes in microglia activity of rat brain induced by
Macrovipera lebetina obtusa venom

Anna A. Darbinyan, Marina V. Antonyan, Hasmik R. Koshatashyan, Susanna S. Gevorgyan,
Hovhannes V. Arestakesyan, Zaruhi I. Karabekian, Naira M. Ayvazyan, Armen V. Voskanyan

Orbeli Institute of Physiology, National Academy of Sciences, Yerevan 0028, Armenia.

Correspondence to: Dr. Armen V. Voskanyan, Orbeli Institute of Physiology, National Academy of Sciences, Yerevan 0028, Armenia.
E-mail: arminvosking@gmail.com
How to cite this article: Darbinyan AA, Antonyan MV, Koshatashyan HR, Gevorgyan SS, Arestakesyan HV, Karabekian ZI, Ayvazyan
NM, Voskanyan AV. Changes in microglia activity of rat brain induced by Macrovipera lebetina obtusa venom. Neuroimmunol
Neuroinflammation 2018;5:41. http://dx.doi.org/10.20517/2347-8659.2018.33

Received: 8 Jun 2018 First Decision: 27 Aug 2018 Revised: 7 Sep 2018 Accepted: 10 Sep 2018 Published: 29 Sep 2018
Science Editor: Athanassios P. Kyritsis Copy Editor: Yuan-Li Wang Production Editor: Huan-Liang Wu

ABSTRACT
Aims: The microglia activity of rat brain following exposure of the Macrovipera lebetina obtusa venom was investi-
gated.

2+
Methods: Histochemical analysis of brain microcirculatory bed staining by Ca ATPase method for variable doses
after intraperitoneal injections given for different time periods was used. The hemorrhagic activity of snake venom
metalloproteinases was tested. Toxicological data were calculated using Behrens and Miller-Tainter methods.
Surface, size of brain microglial cells (MGCs) and staining intensity were quantified using ImageJ software.

Results: The vasodestructive action of the venom resulted in changes in ATPase activity. The intensity of staining of
rat brain microcirculatory bed was venom dose-, and time-dependent. Increased activity of MGCs in hemorrhagic
loci of different regions of venom affected brain was also demonstrated.

Conclusion: The activation of microglia and changes of its form, size, and position strongly correlates with
hemorrhage-induced cerebrovascular damage.

Keywords: Macrovipera lebetina obtusa venom, toxicity LD 50 , hemorrhage, metalloproteinase, rat brain
microcirculatory bed, microglia

© The Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0
International License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use,
sharing, adaptation, distribution and reproduction in any medium or format, for any purpose, even commercially, as long
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