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Mondal et al. Neuroimmunol Neuroinflammation 2018;5:34 Neuroimmunology
DOI: 10.20517/2347-8659.2018.13 and Neuroinflammation

Original Article Open Access

The novel-molecule T11TS facilitated arousal
of glioma-mediated dormancy of bone-marrow

hematopoietic stem-cells

Somnath Mondal , Ankur Datta , Iman Hazra , Sk Md Omar Faruk , Saibal Moitra , Suhnrita Chaudhuri ,
1,2
1
1
3
1,2
1
Lakshyajeet Nath , Prasanta Kumar Das , Anjan Kumar Basu , Santanu Kumar Tripathi , Swapna Chaudhuri 1
4
1
1
2
1 Department of Laboratory Medicine, Calcutta School of Tropical Medicine, Kolkata 700073, West Bengal, India.
2 Department of Clinical & Experimental Pharmacology, School of Tropical Medicine, Kolkata 700073, West Bengal, India.
3 Centre for Tumour Biology,Barts Cancer Institute, Queen Mary University of London, Charterhouse Square, London EC1M, UK.
4 Department of Biochemistry and Medical Biotechnology, School of Tropical Medicine, 108 C. R. Avenue, Kolkata 700073, West
Bengal, India.
Correspondence to: Prof. Swapna Chaudhuri, Department of Laboratory Medicine, Calcutta School of Tropical Medicine, 108 C.
R. Avenue, Kolkata 700073, West Bengal, India. E-mail: swapna.chaudhuri@gmail.com
How to cite this article: Mondal S, Datta A, Hazra I, Omar Faruk SM, Moitra S, Chaudhuri S, Nath L, Das PK, Basu AK, Tripathi
SK, Chaudhuri S. The novel-molecule T11TS facilitated arousal of glioma-mediated dormancy of bone-marrow hematopoietic
stem-cells. Neuroimmunol Neuroinflammation 2018;5:34. http://dx.doi.org/10.20517/2347-8659.2018.13
Received: 27 Mar 2018 First Decision: 14 May 2018 Revised: 10 Aug 2018 Accepted: 14 Aug 2018 Published: 30 Aug 2018
Science Editor: Athanassios P. Kyritsis Copy Editor: Huan-Liang Wu Production Editor: Cai-Hong Wang

Abstract
Aim: T11TS, a potent anti-gliomagenic glycoprotein, stimulates both peripheral and intracranial immune response.
The status of bone marrow hematopoietic stem cells (BMHSCs), the cradle of regeneration of all blood cells, during
gliomagenic global immune devastations has not yet been investigated. Therefore, we aimed to delineate the effects of
T11TS on immature and mature compartments of hematopoietic machinery.

Methods: Flowcytometric analysis of cultured BMHSCs was evaluated for assesing the expression pattern of early
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hematopoietic stem cells (HSCs) markers such as CD34 , Sca-1 , c-kit and also Angiopoietin-1 and Tie-2 both in normal,
glioma, and in T11TS treated glioma-bearing animals. Immunofluresenece imaging and western blot analyses of BMHSCs
were also carried out.
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Results: There was significant downregulation of HSCs-markers CD34 , Sca-1 , c-kit in ethyl nitrosourea-induced glioma-
bearing animals followed by an increase in the expression level of Ang-1 and Tie-2 that determines the quiescence and
self-renewability of stem cells. T11TS administration reversed the gliomagenic transformation of expression of the above
mentioned markers. The results flowcytometric-analysis was also well corroborated with immunofluorescence imaging
and western blot analysis.

© The Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0
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