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Sharma et al. Zinc supplementation prevents against LPS induced neurotoxicity in rats

Figure 3: Representative figures are HE stained coronal sections of the brain showing the effect of prenatal zinc supplementation in
hippocampal region of prenatally LPS treated (A) male and (B) female pups. Left side: ×10; right side: ×40. LPS: lipopolysaccharide
supplementation against LPS-induced endotoxemia Prenatally LPS treated male and female pups spent
during third trimester of pregnancy were investigated. maximum time in open arm on EPM and they escaped
It was observed that LPS-induced endotoxicity has maximum number of trails in active avoidance
major effects on female pups as compared to male test performed in the present study. This aberrant
pups. Although behavioral impairments were seen behavior can be due to loss of memory. Several
in both prenatally exposed male and female pups. reports demonstrated different memory based tests
Importantly prenatal zinc supplementation prevented like nest-seeking, cliff avoidance and morris water
these effects in female pups. Many studies have maze etc. on prenatally LPS treated pups and pups
reported behavioral impairments in new born as well showed impairment in behavior. [51,52] Maternal zinc
as adult off-spring due to early, mid or late gestational supplementation prevents aberrant behavior in object
[49]
LPS exposure. According to Taweel et al., it was recognition task in mice off spring following early
gestational exposure of LPS. In accordance with
[53]
shown that gestational exposure of LPS has an the previous reports in case of present study also
inhibitory effect on righting reflex, rotating reflex and an improvement in memory following maternal zinc
cliff avoidance activity in mice off-spring (PND21). Also, supplementation was observed.
zinc supplementation during pregnancy significantly
improved reduced motor activity and responsiveness Loss of total locomotor activity and short term memory
in rats and monkeys. Similarly, in the present were further correlated with decrease in dopamine
[50]
experiment with zinc supplementation to mothers (DA) and serotonin (5-HT) levels. As per previous
throughout pregnancy showed improvement in total reports a long term reduction of DA and 5-HT along
locomotor activity as well as rotarod behavior. with other neurophysiological changes were observed
40 Neuroimmunology and Neuroinflammation ¦ Volume 4 ¦ March 21, 2017