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Ding et al. J Transl Genet Genom 2021;5:50-61 I http://dx.doi.org/10.20517/jtgg.2020.01 Page 55

Table 3. Function and pathway annotation of 36 genes in the iPAM classifier
Immune Cell adhesion
Gene Bone-disease Cell Gene function, disease association, and pathway role (abstracted
name * related pathway /cell-matrix cycle from the gene card website, https://www.genecards.org/)
related
related
ANO7 Yes Associated with advanced CaP, a target in CaP diagnosis and
immunotherapy
ANTXR1 Yes Yes TLR Pathway, ECM proteins, actin cytoskeleton and promotes cell spreading
ASPN Yes Yes Degradation of extracellular matrix (ECM) proteoglycans, associated with
Osteoarthritis
ATP5EP2 Purine nucleotides de novo biosynthesis
AZGP1 Yes Member of macroglobulin family; antigen binding; validated predictor of
metastatic CaP
C7 Yes Pathway of the innate immune system; involved in host immunity
CCDC6 Structural constituent of cytoskeleton, associated with thyroid papillary
carcinoma
CD24 Yes Modulates growth and differentiation signals to granulocytes and B cells
CDC42EP5 Yes Induces actin filament assembly leading to cell shape changes.
CYBA Yes TNFR1 Pathway and Class I MHC mediated antigen processing and
presentation
DDIT4 Yes DDIT4/mTOR axis involved in the differentiation of Th17 cells
DPT Yes Cell-matrix interactions and matrix assembly, mediate adhesion
FAM13C1 GTPase activator activity
FBXL8 Yes Involved in Class I MHC mediated antigen processing and presentation
GLO1 Yes Risk factor for CaP progression and involved in bone formation and
resorption
GLYATL1 Cytochrome P450 - arranged by substrate type and Conjugation of
carboxylic acids
GMNN Yes Cell cycle regulation, increased expression plays role in colon, rectal and
breast cancer
GNPTAB Yes Associated with mucolipidosis II and IIIA with low bone mineral density
(osteoporosis)
ITGBL1 Yes Participates in cell adhesion as and cell-surface, cell-cell and cell-matrix
interaction.
KIF21A Yes Involved in microtubule transport, involved in cell polarity, and migration
KRT15 Yes Yes Involved in the structural integrity of epithelial cells; regulating innate
immunity
LBH Contributes to Wnt-induced tumorigenesis
LRRN1 Regulates Differentiation of Embryonic Stem Cells
LTF Yes Anti-inflammatory activity, induce apoptosis and inhibit proliferation in
cancer cells.
MTDH Yes Promotes lung metastasis and also has an effect on bone and brain
metastasis
MYBPC1 Yes Contributes to the stability and maintenance of sarcomeres.
NCAPD3 Yes Chromosome condensation in prometaphase and Cell Cycle, Mitotic
NR4A1 Yes Involved in Class I MHC mediated antigen processing and presentation
PCA3 Sensitive to androgen-receptor activation, a molecular signature of prostate
cancer
RNF39 Plays a role in an early phase of synaptic plasticity
SFRP4 Yes Modulators of Wnt signaling, associated with Osteomalacia and Pyle
Disease
SLC22A3 Disposition of small organic cations and drugs and environmental toxins
SLC37A3 Transporter activity and transmembrane transporter activity
STRBP DNA and RNA binding, plays a role in regulation of cell growth
TOP2A Yes Associated with aggressive CaP and drug resistance; Essential in mitosis
and meiosis
UGDH Yes Components of the ECM involved in cell migration, and cancer growth and
metastasis
*ANO7 and MYBPC1 are also in the Decipher classifier [15] ; Top2A is also in the Prolaris classifier [27] ; AZGP1 and SFRP4 are also in the
Oncotype DX classifier [26] ; IPAM: Improved prediction analysis of microarray.

The AUC of survival ROC for the iPAM classifier by Gleason scores and age groups is shown in Table 4. The
highest AUC of 0.87 (95%CI: 0.78-0.94) was observed in young patients (age ≤ 55 years); an intermediate
AUC of 0.82 (95%CI: 0.76-0.88) was observed for middle-aged patients (age 56-70 years), and the lowest
AUC of 0.69 (95%CI: 0.55-0.82) was observed in old patients (age > 70 years) [Figure 3B]. For patients with
Gleason scores of 7-10, the overall AUC was 0.80 (95%CI: 0.74-0.85), with the highest AUC of 0.85 (95%CI:
0.75-0.93) in the young group and the lowest AUC of 0.67 (95%CI: 0.50-0.80) in the old group. The iPAM