Page 227 - Read Online

Park et al. J Cancer Metastasis Treat 2019;5:17 Journal of Cancer
DOI: 10.20517/2394-4722.2018.84 Metastasis and Treatment

Original Article Open Access

AF1q inhibited T cell attachment to breast cancer
cell by attenuating Intracellular Adhesion Molecule-1

expression

Jino Park 1,2,# , Jae Yeon Hwang , Alexandra Thore , Soojin Kim , Tomiteru Togano , Shotaro Hagiwara ,
2,4
1,2
5
3,#
1,2
Juw Won Park , William Tse 1,2
3
1 James Graham Brown Cancer Center, University of Louisville School of Medicine, Louisville, KY 40202, USA.
2 Division of Blood and Bone Marrow Transplantation, Department of Medicine, University of Louisville School of Medicine,
Louisville, KY 40202, USA.
3 Department of Computer Science and Computer Engineering, University of Louisville, Louisville, KY 40292, USA.
4 Division of Haematology, National Center for Global Health and Medicine, Tokyo 162-8655, Japan.
5 Division of Haematology, Tokyo Women’s Medical University, Tokyo 162-8666, Japan.
# Both authors contributed equally to this work.
Correspondence to: Dr. Jino Park, CTRB 342A, 505 South Hancock street, Louisville, KY 40241, USA.
E-mail: jino.park@louisville.edu

How to cite this article: Park J, Hwang JY, Thore A, Kim S, Togano T, Hagiwara S, Park JW, Tse W. AF1q inhibited T cell attachment
to breast cancer cell by attenuating Intracellular Adhesion Molecule-1 expression. J Cancer Metastasis Treat 2019;5:17.
http://dx.doi.org/10.20517/2394-4722.2018.84

Received: 1 Dec 2018 First Decision: 30 Dec 2018 Revised: 15 Jan 2019 Accepted: 24 Jan 2019 Published: 18 Mar 2019

Science Editor: William P. Schiemann Copy Editor: Cai-Hong Wang Production Editor: Huan-Liang Wu

Abstract
Aim: To investigate whether AF1q, overexpressed in metastatic cells compared with the primary tumor cells, plays
a pivotal role in breast cancer metastasis.

Methods: To investigate whether AF1q has a responsibility in the acquisition of a metastatic phenotype, we
performed RNA-sequencing (RNA-Seq) to identify the gene signature and applied the Metacore direct interactions
network building algorithm with the top 40 amplicons of RNA-Seq.

Results: Most genes were directly linked with intercellular adhesion molecule-1 (ICAM-1). Likewise, we
identified that ICAM-1 expression is attenuated in metastatic cells compared to primary tumor cells. Moreover,
overexpression of AF1q attenuated ICAM-1 expression, whereas suppression of AF1q elicited the opposite effect.
AF1q had an effect on ICAM-1 promoter region and regulated its transcription. Decreased ICAM-1 expression

© The Author(s) 2019. Open Access This article is licensed under a Creative Commons Attribution 4.0
International License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use,
sharing, adaptation, distribution and reproduction in any medium or format, for any purpose, even commercially, as long
as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license,
and indicate if changes were made.

www.jcmtjournal.com