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Inter-sample correlations

D283-C D283-M D341-C D341-M DAOY-C DAOY-M TB0011-MB TB0012-CTR2 TB0012-CTR1
D283-C 100.00% 21.69% 85.12% 20.54% 83.91% 17.33% 19.55% 20.36% 19.58%
D283-M 21.69% 100.00% 23.70% 91.94% 29.50% 83.96% 88.54% 88.77% 86.66%
D341-C 85.12% 23.70% 100.00% 22.64% 93.61% 18.88% 20.34% 22.13% 20.65%
D341-M 20.54% 91.94% 22.64% 100.00% 28.22% 86.76% 85.53% 85.12% 83.75%
DAOY-C 83.91% 29.50% 93.61% 28.22% 100.00% 23.71% 26.54% 26.54% 27.26%
DAOY-M 17.33% 83.96% 18.88% 86.76% 23.71% 100.00% 93.56% 93.56% 91.32%
TB0011-MB 19.55% 88.54% 20.34% 85.53% 26.54% 93.56% 100.00 98.02% 97.37%
TB0012-CTR2 20.36% 88.77% 22.13% 85.12% 28.05% 92.88% 98.02% 100.00% 98.43%
TB0012-CTR1 19.58% 86.66% 20.65% 83.75% 27.26% 91.32% 97.37% 97.37% 100.00%

Intra-and inter-type correlations :

Cell lines CM CSF-MB CSF-CTR

Cell lines 87.59% 22.91% 22.14% 23.01%
CM 22.91% 87.55% 93.56% 88.08%

CSF-MB 22.14% 93.56% 100.00% 97.69%

CSF-CTR 23.01% 88.08% 97.69% 98.43%
a

b
Figure 3: (a) Pearson correlation analysis for indicated samples; (b) PCA graph representing microRNA spectra inside MB cell lines (cell line name-C), in culture medium
(cell line name-M) and in CSF of MB patient (TB0011-MB) compared to control TB0021_CTR1 and TB0012_CTR2. Graph demonstrating similarity between miRNA
profi le in CM and those secreted in CSF of MB patient. CM: Culture medium; PCA: Principal component analysis; MB: Medulloblastoma; CSF: Cerebral spinal fl uid

neurological disease). PCA [Figure 2] showed clear controls [Figure 4c] and [Supplementary Table 5]. Further
separation between the miRNA spectrum in CSF of analysis identifi ed 268 miRNAs over-represented (with
MB patient and controls. Microarray analysis identifi ed fold-change > 2) and 6 miRNAs under-represented in
1,254 miRNAs in the MB-CSF sample [Table 1], MB-CSF compared with the 2 different controls tested
of which 86 miRNAs were differentially expressed [Supplementary Tables 6 and 7], indicating a trend
in CSF of the MB patient compared to the 2 CSF toward miRNA enrichment in the MB-CSF sample.
70 Journal of Cancer Metastasis and Treatment ¦ Volume 1 ¦ Issue 2 ¦ July 15, 2015 ¦