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Page 4 of 13 Udukala et al. J Cancer Metastasis Treat 2020;6:25 I http://dx.doi.org/10.20517/2394-4722.2020.45
Scheme 1
Figure 2. Fe/Fe 3 O 4 -based nanobiosensor for matrix metalloproteinase 1 detection. Consensus sequence GAGVPMS-MRGGAG. TCPP:
tetrakis-carboxyphenyl-porphyrin. Scheme 1. A: Design principles of a nanobiosensor for protease detection. Consensus sequence:
GAGVPMS-MRGGAG (MMP1); The average distance between fluorophore TCPP (tetrakis-carboxyphenyl-porphyrin) and Fe/Fe 3 O 4
nanoparticle and FRET-acceptor cyanine 5.5 increases upon cleavage of the oligopeptide tether by a suitable protease, leading to an
increase in fluorescence; B: TEM and HRTEM of dopamine-coated Fe/Fe 3 O 4 core/shell nanoparticles; C: Typical emission spectra
o
occurring from the nanosensor for MMP 13 after 1 h of incubation at 37 C (λ exc = 421 nm). low: buffer; middle:nanosensor; high:
[4]
nanosensor after incubation with MMP13 .
